Experimental Research of Foam SiC in Repairing Mandibular Critical-sized Defects of Rabbits
|School||China Medical University|
|Keywords||Bone repair materials SiC foam Critical bone defects Hard tissue sections|
The purpose of tumors, inflammation, trauma causes a large area of ??mandibular defects caused not only give patients different degree of of physiological deformities and loss of function, but also cause severe psychological trauma. Since there are obvious shortcomings autologous bone allograft, the development of three-dimensional porous bioactive artificial bone geometry features of trabecular bone is an important task in the field of medical and biological materials. Hydroxyapatite (hydroxyapatite, HA) porous biomimetic materials on behalf of its good biocompatibility has been confirmed by a large number of experimental and clinical applications, HA mechanical properties and complex geometries accurately weak manufacturing capacity make it difficult to complete a large area and complex shape bearing bone defects. The new SiC foam using \good biocompatibility, the experimental by rabbit mandibular critical bone defects artificial materials implanted in the animal model to observe the effect of contrast the foam SiC and HA in mandibular critical bone defects, to explore this new type of foam silicon carbide as The feasibility of bone repair materials to further study its biological characteristics. Method 1, the establishment of animal models choose healthy adult Japanese white rabbits 28, 24 of them produced by the Department of the critical bone defects in bilateral mandibular body (13mm x 6mm x 4mm) side of the implantation of the experimental material SiC foam (SiC), the other side of the implantation of hydroxyapatite (HA) for self-control, and another four making unilateral mandibular critical bone defects as a blank control. 2, drawn drawn the week before the injection of tetracycline hydrochloride (25mg/kg), respectively, after 4 weeks, 8 weeks, 24 weeks. Blank control group at 24 weeks after drawn. 3, producers gradient alcohol dehydration, methyl methacrylate-embedded, LEIKA-SP1600 main saw slicing machine perpendicular to the specimen long axis slices, select the middle sections were observed under fluorescent microscope and HE staining and toluidine blue staining. 4, the observed line animal general observation, and the gross observation fluorescence microscopy tetracycline marked new bone formation was observed under an optical microscope HE staining and toluidine blue stained sections of bone formation and selection of different multiples radiography. 5, the computer image analysis to select each specimen two middle slices, each slice randomly selected three fields. Fluorescent tetracycline labeling rate tetracycline marking surface of each field of vision (× 40) percentage of total bone surface (%). The osteoblasts area HE staining analysis, to represent the average value (μm ~ 2) within a field of view (× 100) into the bone area. Fluorescent tetracycline labeling rate application Metamorph Imaging System V4.5 image analysis software and osteogenic area estimated, tetracycline labeling rate of SPSS 11.0 software statistical analysis of the experimental group and the control group, and osteogenesis area without significant difference. Results of experimental animals in good health, the wound healed; specimens see various sampling time points the foam SiC and HA material surrounding its intranet hole have varying degrees of bone tissue formation was observed; stereo microscope clearly observed material pore within the new bone tissue ingrowth; histology observed in the repair of bone tissue is a normal bone repair process; statistical analysis showed that tetracycline fluorescence labeling rate of four weeks after the foam SiC and osteogenic area below the HA and the difference was statistically significance (P lt; 0.01, P lt; 0.05), while no significant difference of the experimental and control groups 8 and 24 weeks after surgery. Conclusion bubble SiC repair the rabbit jaw critical bone defects long-term effect similar to that of HA, has good bone conduction, coupled with superior mechanical properties and processing performance, which is expected to become the new complex shape and a large segment of the load-bearing bone defect repair porous the bionic biological material.