Dissertation > Medicine, health > Internal Medicine > Systemic disease > Poisoning and chemical damage > Alcohol poisoning

APP and Aβ Protein Expression in Mice Neurons with Chronic Alcoholism

Author HuZuo
Tutor ZhangGuoHua
School China Medical University
Course Forensic
Keywords Chronic alcoholism Alzheimer's disease Amyloid precursor protein β-amyloid peptide
CLC R595.6
Type Master's thesis
Year 2009
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Introduction China is a populous country, and is a process of aging the fastest aging population in many countries. China is a historic diverse wine culture coexist country. Alcoholism often occurs due to the special social and historical reasons. Chronic alcoholism (chronic alcoholism) in ethanol addiction is characterized by neurological symptoms appear when its temperance, often repeated drinking ethanol tolerance, withdrawal (withdrawal). Chronic alcoholism caused serious damage in the nervous system and other organs, including the most serious damage to the nervous system. Forensic practice sometimes encountered cases of alcoholism, often associated with traffic accidents, an important organ and tissue dysfunction. Alzheimer's disease (Alzheimer's disease, AD) is a progressive neurodegenerative disease, comprehensive cognitive dysfunction. The main pathological changes of amyloid plaques formed by β-amyloid peptide (beta-amyloid, Aβ) derived from amyloid precursor protein (amyloid precursor protein, APP) and excessive or abnormal phosphorylation of Tau protein neurofibrillary tangles. The pathogenesis of Alzheimer's disease is affected by many factors, including environmental factors, poor eating habits, types of drug poisoning. Chronic alcoholism cause brain APP and Aβ protein causing the occurrence of Alzheimer's disease, is still a lack of systematic theoretical and experimental study. Neuron specific enolase (Neuron-specific enolase, NSE) is a symbol of the nerve cell protein, the presence of specific neurons and neuroendocrine cells, accounting for 1.5% of all soluble brain protein, neuronal damage or The necrosis NSE into the bloodstream. In this study, in mouse chronic alcoholism on the basis of animal models, application histopathology and immunohistochemistry techniques, studies in mice with chronic alcoholism, APP and Aβ in the brain tissue of nerve cells expression and SPSS statistical software Statistical analysis was performed to explore the APP and Aβ content changes in the nerve cells in the brain of chronic alcoholism, and the pathogenesis of Alzheimer's disease a causal relationship between the observed experimental results. Materials and Methods, the establishment of animal models and grouped male health 9 weeks 42 Kunming mice were randomly divided into two 30-day group, 60 days group, and each group was divided into 10% alcohol group, 20% alcohol group and the control group, control group five, eight of the 10% alcohol group, 20% of the alcohol groups of eight each. Experimental group anhydrous ethanol (HPLC grade) with mineral water for drinking dubbed the concentration of 10%, 20% alcohol solution as the drinking water of laboratory mice, the control group drinking mineral water instead. After the end of the experiment, direct decapitated and the animals quickly extract the brain tissue of mice, 4% paraformaldehyde / PBS pH7.4 were fixed, paraffin-embedded, producer. Neuron-specific brain cells after the morphological changes of the histopathology of the nerve cells in the experimental method (1) HE staining was observed in mice with chronic alcoholism; (2) using immunohistochemical staining was observed in mice with chronic alcoholism enolase expression changes; (3) using immunohistochemical staining was observed in mice with chronic alcoholism neuronal intracellular expression of APP and Aβ; (4) Motic Images Advanced 3.2 image analysis system using immunohistochemical staining cells The number of cells positive reaction product of optical density; (5) Application of SPSS statistical software for statistical analysis of the experimental data. Experimental results (1) experimental mice chronic poisoning performance, and body weight decreased significantly; (2) HE staining in chronic alcoholism mouse brain tissue edema, nerve cells Nissl bodies disappear or margination nerve cells around the gap widened; (3) neuron-specific enolase immunohistochemical staining, see an increase in enzyme protein content; (4) the control group, the nerve cells of the small amount of APP immunohistochemistry positive staining product distribution in cell extracellular brownish yellow pulp. Experimental group APP positive cells increased significantly, enhanced expression of different time periods and different concentrations of positive expression of varying intensity. Appear obvious of Aβ positive staining product within the cytoplasm of nerve cells in the brain of the experimental group, and high concentrations of prolonged administration group the number of positive cells increased significantly enhanced expression. Motic Image Advanced 3.2 image analysis system to calculate the mean integrated optical density, and statistical analysis using SPSS software, found a significant difference between the experimental and control groups. Conclusion 1. Chronic alcoholism can cause the nerve cells in the brain of laboratory mice showed degenerative changes; chronic alcoholism can cause nerve cells in the brain of laboratory mice nerve yuan increase in specific enolase and abnormal distribution; 3. Chronic alcoholism can cause experimental mice produce Aβ protein in the cytoplasm of nerve cells in the brain, and the APP protein expression was significantly increased. Chronic alcoholism may be one of the relevant factors induce the onset of Alzheimer's disease.

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