Alkaline Phosphatase Activity in Gingival Tissues of Experimental Periodontitis in Rats
|Keywords||Alkaline phosphatase Periodontitis Correlation|
Objective: To study alkaline phosphatase (alkaline phosphatase, ALP) in rats with experimental periodontitis gingival tissue content and distribution , and periodontitis correlation . : Successfully established model of experimental periodontitis in rats respectively modeling four weeks and were sacrificed at 8 weeks after surgery . Remove modeling at the gum tissue was determined using a microplate reader ALP content of rat gingival tissue ; tissue sections of ALP Gomori modified Gomori production and distribution of alkaline phosphatase in gingival tissue sections in the light microscope . The results : 1 . Alkaline phosphatase in the gum tissue changes in the content of ALP in modeling after 4 weeks and 8 weeks periodontitis group gum tissue was significantly higher than the normal control group ( P <0.01 ) . ALP in gingival tissue in periodontitis group , modeling group after 8 weeks were significantly higher than four weeks after surgery group (P <0.01). 2 . Alkaline phosphatase in the gingival tissue distribution of ALP in the modeling after 4 weeks and the distribution density of the gingival tissue sections of 8 weeks periodontitis group was significantly higher than the normal control group . Modeling periodontitis group 8 weeks after surgery gum tissue slice distribution density of the ALP was significantly higher than four weeks after the group . Conclusion: In this study, through the establishment of animal models of experimental periodontitis observed : periodontitis group modeling postoperative 4 weeks significant periodontal tissue destruction , the ligated teeth near and far , and the root bifurcation of the alveolar bone resorption in the gum tissue the ALP content also significantly higher than the normal control group . Modeling eight weeks after periodontal tissue damage is more significant , ligation of the teeth as well as the adjacent teeth alveolar bone absorption , showing anti- wave -shaped defect in the gum tissue ALP content was significantly higher than four weeks after . Thus , the content of the ALP in the experimental periodontitis group gum tissue was significantly higher than the normal control group , and its content was significantly higher with the progress of periodontitis , suggesting that the content of the gum tissue ALP periodontitis the progress of a positive correlation between .