Reconstruction of Corneal Surface by Autologous Transplantation of Rabbit Adipose Tissue-derived Stem Cells on Amniotic Membrane
|Keywords||Tissue Engineering Limbal stem cell defect Corneal epithelial Fat stem cells Autologous transplantation|
[Objective ] through in vitro tests of rabbit adipose stem cells on amniotic growth . Autologous fat stem cells , in vivo experimental observation reconstruction of the corneal epithelium , and to provide new ideas for the clinical treatment of patients with limbal stem cell defect . [ Method ] In vitro: The conventional method to obtain rabbit adipose stem cells , amniotic membrane as a carrier , the growth of adipose stem cells on amniotic HE staining , scanning and transmission electron microscopy . In vivo experiments : the establishment of rabbit limbal stem cell defect model , the experimental group divided into simple model group , amniotic membrane transplantation group , adipose-derived stem cells in amniotic membrane transplantation group , the use of fluorescent staining , confocal microscopy , HE staining , immunohistochemistry chemical dyed checks , and scanning and transmission electric mirror inspection and evaluation of adipose stem cells on corneal epithelial defect repair . [ Results ] fat stem cells in the amniotic membrane growth well attached . Corneal staining , HE staining showed that the fat stem cells on amniotic membrane transplantation group more effective than the other group , the treatment of limbal stem cell defect confocal microscope and electron microscope examination showed the growth of fat stem cells on the surface of the cornea . Fat stem cell renewal corneal epithelial immunohistochemical staining K3/K12 negative expression , suggesting that fat stem cells by in vivo induction and can not be converted to the corneal epithelium . [ Conclusion ] fat stem cells as seed cells , amniotic membrane as a carrier can better reconstruction of the corneal epithelium , which provide new ideas for the clinical treatment of patients with limbal stem cell defect .