The Application of CD-DST in Head and Neck Squamous Cell Carcinoma
|School||Tianjin Medical University|
|Keywords||Head and neck cancer Squamous cell carcinoma Chemosensitivity Primary cell culture Collagen gel embedded chemosensitivity testing Cisplatin Notch1|
Objective: The primary tumor cells in collagen gel embedded chemosensitivity testing technology (Collagen gel dropletembedded culture-drug sensitivity test, CD-DST) is a state-of-the-art chemotherapy drug susceptibility testing technology can accurately predict the chemosensitivity of the study by CD-DST detection of head and neck squamous cell carcinoma and sensitivity difference of five kinds of chemotherapy drugs used in clinical, explore the CD-DST in head and neck squamous cell carcinoma in the clinical application value. And CD-DST technology for head and neck squamous cell carcinoma of the basic research to explore Notch1 expression levels and activity in head and neck squamous cell carcinoma sensitivity to cisplatin. Method: a collection of Tianjin Medical University Cancer Hospital from January 2007 to September 2008, diagnosis and treatment of 28 patients with head and neck squamous cell carcinoma of fresh surgical specimens, the use of CD-DST method detected five kinds of chemotherapy drugs (paclitaxel, cisplatin, 5-FU, bleomycin, vinorelbine) sensitivity. Analysis of different gender, age, lymph node status, T stage, histological grade and intraoperative chemosensitivity difference between the pre-operative chemotherapy; relationship between the analysis of preoperative chemotherapy and postoperative chemotherapy drug sensitivity. 2 collection Tianjin Tumor Hospital, diagnosis and treatment of 25 cases of fresh surgical specimens archived paraffin block specimens from January 2007 to May 2008; CD-DST technology detection sensitivity to cisplatin; tongue squamous cell carcinoma of the recovery cell lines Tb3.1, the parallel cells climbing film; the immunochemical staining Head and neck squamous cell carcinoma, the expression level of the normal squamous epithelium and tongue squamous cell carcinoma cell line (TB3.1) Notch1; by DMSO, DAPT respectively after TB3.1 cells were divided into four groups (DMSO group, the DAPT group of, DMSO DDP group, DAPT DDP group), after the to by 2μg/ml cisplatin, the absorbance value of the CD-DST detection, analysis on the role of DAPT TB3.1 cell sensitivity to cisplatin. Results: 1 CD-DST detection success rate is higher, 89.3% (25/28); patients with head and neck squamous cell carcinoma there are individual differences in sensitivity to chemotherapeutic drugs. Chemosensitivity of different gender, age, lymph node status, T stage, histological grade and whether surgery was no significant difference between the pre-operative chemotherapy (P> 0.05); head and neck squamous cell carcinoma patients five kinds of chemotherapy-sensitive drugs, in descending order of paclitaxel (40%), cisplatin (32%), 5-Fu (32%), bleomycin (24%), vinorelbine (20 %), and clinical reports basically consistent with efficiency; related to the response to preoperative chemotherapy and postoperative CD-DST results (r = 0.624, P <0.05). 2 CD-DST detection cisplatin effective rate of 32% efficiency (27% -40%) consistent with clinical reports; Notch1 expression of head and neck squamous cell carcinoma, the positive part in the cytoplasm, expression levels were significantly higher than normal squamous epithelium (P <0.001), and Notch1 expression level of sensitivity to cisplatin was negatively correlated (r = -0.705, P <0.01); of TB3.1 cells Notch1 expression, positive parts in the cytoplasm, DMSO group (155.4 ± 2.3) with DAPT group (154.7 ± 1.2) absorbance value was no significant difference (P> 0.05) of DMSO DDP group (21.1 ± 1.3) and DAPT the DDP group of (26.6 ± 1.1) absorbance values ??with significant difference (P <0.05). Conclusion: 1 CD-DST detection success rate, head and neck squamous cell carcinoma sensitivity to chemotherapy there was a significant individual differences. Gender, age, lymph node status, T stage, histological grade and whether preoperative chemotherapy sensitivity to chemotherapeutic drugs had no effect; CD-DST detection can provide guidance to individual chemotherapy for head and neck squamous cell carcinoma. High expression of Notch1 in head and neck squamous cell carcinoma, and the expression level of sensitivity to cisplatin was negatively correlated, Notch1 can be used to predict the head and neck squamous cell carcinoma cisplatin sensitivity; DAPT blocking Notch1 signaling pathway can improve TB3.1 cell sensitivity to cisplatin.