The Experimental Study on Quantizing Dominant Wet and Dominant Heat Type of the Spleen and Stomach Wet Heat Syndrome
|School||Southern Medical University,|
|Course||Chinese and Western medicine combined with the basis|
|Keywords||Spleen Wet emphasis Hot emphasis San Ren Tang White Tiger add herb soup Animal Model|
Objective Spleen is a common clinical syndromes, their presence in the disease process wet emphasis, hot and humid both emphasis and other pathological changes in the clinical diagnosis more difficult. This issue through the general state of the rats, urinary aquaporin 2 (AQP2), plasma lymphocyte heat shock protein 70 (HSP70), antral substance P (SP) and somatostatin (SS), stomach pathological changes in the duodenum and other indicators to explore Spleen wet, hot emphasis on nature and the differences between them, as Spleen wet, hot emphasis on quantitative and provide experimental evidence; through observation in San Ren Tang, White Tiger plus herb soup intervention Spleen wet emphasis, thermal conditions and the general emphasis rats indicators of changes in a series of experiments, from different angles reveals three Ren Tang, White Tiger add herb soup mechanism of action. Method 1. Drugs and feed ① aconite dried ginger of drugs: aconite, ginger ② Sanren soup Ingredients: bitter almond, France Pinellia, talc, Coix seed, white cardamom, Magnolia, through the grass, light bamboo ③ White Tiger add herb soup Ingredients: herb, rice, Anemarrhena, gypsum, licorice. High-sugar high-fat diet: normal diet mixed with 12% lard, 8% honey processing. (2) grouping 40 SPF SD rats were randomly divided into five groups of eight, male and female, that the normal control group, wet emphasis group, San Ren Tang Group, hot emphasis on group and tiger add herb soup group. 3 modeling in fatness diet, alcohol injury, hot and humid environment, pathogenic microorganisms combination of factors, the establishment of Spleen wet emphasis on type, thermal emphasis on animal models of type: ① normal control group: 20 ~ 28 ℃ temperature , 50% to 70% relative humidity environment, an ordinary mixed diet; ② wet emphasis Group: High-fat diet sugar liquor box modeling E. coli; ③ San Ren Tang Group: High-fat diet sugar liquor box modeling E. San Ren Tang; ④ Heat emphasis groups: high glucose and lipid box modeling aconite ginger decoction of E. coli; ⑤ White Tiger add herb soup group: high glucose and lipid box modeling aconite decoction E. White Tiger plus the herb ginger soup. 4 observation and detection index contrast study in rats before and after modeling the general state, weight, rectal temperature, 24 hours urine, stool and other changes. By ELISA rat urine AQP2, plasma cells by flow cytometry HSP70, radioimmunoassay antrum homogenates of SP and SS content. Light microscope rat stomach, duodenum pathological changes. 5. Statistical methods using SPSS software (version 13.0) treatment, the experimental results of the data type for the measurement data, the data are presented as mean ± standard deviation ((?) ± s) expressed. Weight, body temperature using repeated measures analysis of variance to compare; 24 hours urine output, AQP2 levels, HSP70, SP and SS were analyzed using One-Way ANOVA results for each group analysis of variance, multiple comparisons between groups using LSD method tests to P <0.05 was considered statistically significant. Results 1. General state of rats in control group: This group rats fed four weeks period, a coat of gloss, good appetite, normal activities, normal stool. Wet emphasis group, hot emphasis Group: With the modeling time, most of the animals appeared tired, addicted to lying lazy move, unresponsive, shaggy, color withered; modeling the first seven days from the start, there were loose stools or diarrhea sticky, wet emphasis were more serious; into the box after modeling, loose stools and diarrhea symptoms get worse or sticky, gradually see perianal filthy. San Ren Tang Group, White Tiger add herb soup group: Rats in the model group after the same symptoms, the San Ren Tang, White Tiger add herb soup gavage, the symptoms have abated. (2) body weight, body temperature, urine output changes over 24 hours of modeling time, enter the modeling box model group after four weight loss, the model group at different time points were lower than the normal weight control group (P <0.01) by San Ren Tang, White Tiger add herb soup weight returned to normal after treatment, the level of growth. Model rats modeling the box after entering the body temperature rises, fed with E. coli after the sharp rise in body temperature, and the normal control group, the difference was statistically significant (P <0.05); through San Ren Tang, White Tiger add herb soup after treatment body temperature returned to normal, and wet emphasis group, hot emphasis on group no significant improvement in body temperature. Wet emphasis group, hot emphasis on group of 24-hour urine output compared with normal control group was significantly decreased (P <0.01), San Ren Tang Group, White Tiger add herb soup group of 24-hour urine output compared with the normal control group, the difference was not statistically significant difference (P> 0.05). 3 wet emphasis group testing laboratory parameters, thermal emphasis AQP2 levels in urine was significantly higher than the control group (P <0.01), and wet emphasis group than in the heat was significantly increased emphasis on group (P <0.01). San Ren Tang Group, White Tiger add herb soup group AQP2 levels returned to normal levels, compared with normal control group showed no significant difference (P> 0.05). Wet emphasis group, hot emphasis on HSP70 expression in plasma cells compared with normal control group (P <0.01), and the emphasis on group than in the hot wet emphasis group had significantly higher (P <0.01). San Ren Tang Group, White Tiger add herb soup group HSP70 expression returned to normal levels, compared with normal control group showed no significant difference (P> 0.05). Wet emphasis group, hot emphasis rats antrum SP, SS compared with normal control group was significantly lower (P <0.01); and wet emphasis group SP, SS levels below the thermal emphasis on group (P <0.05). San Ren Tang Group, White Tiger add herb soup group SP, SS levels returned to normal levels, compared with normal control group had no significant difference (P> 0.05). 4 gastroduodenal pathology layer of the normal control group were few inflammatory cells in the gastric mucosa, submucosa without tissue edema, duodenal villus epithelial more complete. Wet emphasis group, hot emphasis rats stomach and duodenum biopsy no large differences were seen: (1) mucosal layer of a large number of neutrophils, eosinophils infiltration, vascular dilatation and congestion; gastric underlying tissue edema, neutrophils, eosinophils infiltration significantly; (2) duodenal villus epithelial cell shedding seen a large number of inflammatory cells. San Ren Tang Group, White Tiger add herb soup group are visible mucosal layer of sliced ??reduce inflammatory cells, vascular dilatation and congestion free, no submucosal edema, duodenal villus epithelial more complete, only see a small amount of inflammatory cell infiltration. Conclusions The results show that in fatness diet, hot and humid environment, pathogenic microorganisms, wine injury, the wet, the combined effects of heat, it can be successfully replicated Spleen wet emphasis on type, thermal emphasis type in animal models. Wet emphasis group, hot emphasis AQP2 levels in urine increased, and wet emphasis group than in the group had significantly higher heat emphasis, showing its physiological role diuresis outward appearance abnormal; AQP2 as Spleen \\Wet emphasis group, hot plasma cells emphasis HSP70 expression was significantly increased, and the emphasis on group than in the hot wet emphasis group had significantly higher; This suggests that the role of multiple factors in rats, the body is self-regulating, self-protection status, HSP70 can be used as quantitative Spleen wet, hot emphasis on the important indicators. Wet emphasis group, heat emphasis rats gastric SP, SS content decreased, wet emphasis group SP, SS levels even lower heat emphasis group; SP, SS content of gastrointestinal function can objectively reflect the state, which also explains wet emphasis card, hot emphasis on the performance of the clinical evidence digestive symptoms. Wet emphasis group, hot emphasis rats stomach, duodenum inflammatory lesions, suggesting Spleen demonstrated clinical symptoms based on their morphological structure. San Ren Tang, White Tiger add herb soup may exist following effects: 1) can be restored by the complex factors of wet emphasis card, hot emphasis on animal model of general state; 2) regulate urine AQP2 levels, restore normal body outward diuresis function; 3) reduce the thermal stress caused by the increased role of HSP70; 4) regulate the body antrum in SP, SS levels; 5) partially restored model rats stomach, duodenum organizational structure function.