Studies on the Pathogenic Mechanism of Botrytis Cinerea Toxin and Its Inactivation
|School||Shandong University of Technology|
|Course||Biochemistry and Molecular Biology|
|Keywords||Botrytis cinerea Toxin Defense enzymes Passivation Ultrastructure Genomic DNA|
Tomato gray mold (Botrytis cinerea Pers.) Infection caused by Botrytis cinerea is a serious hazard worldwide crop diseases, and its metabolites toxin is the most important pathogenic factor. This paper studies the biological activity of Botrytis cinerea toxins, pathogenic mechanism; screened toxin passivation, a preliminary study and the passivation mechanism. This study provides a new way of thinking for the prevention and control of tomato gray mold has important theoretical and practical significance. Tomato gray mold original bacteria were identified from morphology and molecular biology perspective, to determine the separation of tomato gray mold of the original strains belonging to Botrytis the Trichosporon Gang Botrytis cinerea. The Botrytis cinerea crude toxin biological activity detected using detached leaf dip Chak law and seedling dip Chak law. Toxins can tomato seedlings wilting symptoms, the chlorotic symptoms appear detached leaves with the toxin concentration increases and processing time extended, the more obvious symptoms. Tomato chloroplast membrane permeability and the impact of the four defense enzymes from the Botrytis cinerea crude toxins pathogenic mechanism of toxin. The results showed that the toxin on the extent of the damage of the tomato chloroplast exacerbated as the treatment time; toxins cause tomato leaf MDA content increased, some damage to the membrane; toxins tomato leaves CAT, POD, SOD, PPO four defense enzymes decreased activity, and ultimately lead to a susceptible tomato leaves. Using detached leaves acupuncture mycelial growth inhibition assay and conidia germination inactivation of the 21 tested compounds were screened, screened with strong inactivation of the Botrytis cinerea crude toxins KMnO4, Na2CO3, C6H8O7, H3BO3, K3C6H5O7 five kinds of compounds; KMnO4 with strong inhibition of Botrytis cinerea spore germination, of Na2CO3, C6H8O7, Na2Si03 and, Na2HPO4 five compounds; growth of Botrytis cinerea mycelium has KMnO4, Na2CO3, C2H2O4 strong inhibitory effect of the three compounds. Concentration 0.5L-1 5gL-1Na2CO3, respectively processing tomato gray mold of the original crude toxin measured of different toxins passivation system the dynamic of the four defense enzymes in tomato leaves. The average processing tomato leaves four Na2CO31.5gL-1 Na2CO33gL-1 toxin passivation system defense enzyme activity of the whole water above the sterile water control and toxin treatment, the overall trend consistent with sterile water control, blunt of better. Botrytis cinerea toxin the passivation system can alleviate toxins destruction of tomato leaf cells ultrastructure. Sterile water treatment tomato leaves, the organelles neatly arranged; chloroplast multi fusiform bilayers uniform, orderly lamellar structure; mitochondrial cristae clear, uniform fine matrix; nucleus karyotheca integrity. Tomato leaf tissue toxin treatment appears plasmolysis, the cell wall has been severely damaged; the chloroplast grana layer fuzzy disorders, most of disintegration; chloroplast membrane, mitochondrial membrane has been severely damaged; mitochondrial cristae disappeared vacuolization. The toxins 1.5gL-1Na2CO3 treatment, cell wall occurred slightly wrinkled; chloroplast slightly deformed, the bilayers uniform, complete, grana lamellae reduce, but neat and orderly; mitochondrial part of the ridge disappears, no vacuolization. Botrytis cinerea toxin passivation system can be a certain degree of slow down the damage caused by the toxins of genomic DNA of tomato leaves. Compared with the toxin, the toxin passivation system processing tomato leaves genomic DNA fragmentation bands significantly reduced.