Dissertation
Dissertation > Medicine, health > Pharmacy > Pharmacology > Experimental Pharmacology

The Effects of Pretreatment with Lipid Emulsion on Bupivacaine, Ropivacaine Infusion Induced Cardiac Toxicity in Rats

Author DuYi
Tutor ChengQiao
School Shanxi Medical
Course Anesthesiology
Keywords Intralipid Bupivacaine Ropivacaine Cardiotoxicity ATP
CLC R965
Type Master's thesis
Year 2011
Downloads 29
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Objective: To study intralipid the rat cardiac toxicity caused by the intravenous injection of bupivacaine, ropivacaine, and to explore its mechanism. Methods: SD adult male rats 72 and weighing about 220-250g, were randomly divided into five groups, the control group (A) (n = 8), bupivacaine control group (B group), bupivacaine intralipid group (C), the ropivacaine control group (D group), the ropivacaine fat emulsion group (E), B, C, D, E, and each group is further divided into lethal group (group 1) and two subgroups of the the drawn group (group 2) (each n = 8). Were anesthetized with 10% chloral hydrate the aldehyde 0.3ml/100g abdominal cavity, with three needle electrode needle inserted into the the rat upper limbs and left lower extremity subcutaneous the monitoring standard II lead electrocardiogram (ECG). Surgical separation of the the rat right internal jugular vein, femoral vein juxtaposition effective administration, separation of the left common carotid artery cannulation connected transducer to monitor the mean arterial blood pressure (MAP). (1) A group: 0.9% saline 3ml/kg/min continuous intravenous pump into six minutes after thoracotomy coring; (group 2) B1: 0.9% saline 3ml/kg/min continuous intravenous infusion of 5 minutes, and 0.5% bupivacaine 2mg/kg/min continuous intravenous infusion into to rats heartbeat arrest; (3) B2: 0.9% saline 3ml/kg/min continuous intravenous infusion of 5 minutes, and then 0.5% to cloth ratio The tetracaine 2mg/kg/min continuous intravenous infusion for 1 minute, thoracotomy coring; (4) C1: 20% fat emulsion the injection 3ml/kg/min continuous intravenous infusion 5 minutes, and then 0.5% bupivacaine The 2mg/kg/min continuous intravenous infusion to rats heartbeat cardioplegia; (5) C2 groups: continuous intravenous infusion of 20% fat emulsion injection 3ml/kg/min five minutes, then 0.5% bupivacaine 2mg/kg / min continuous intravenous infusion into one minute, thoracotomy coring; (6) D1 group: 0.9% saline 3m / kg / min continuous intravenous pumped five minutes, and then 0.5% ropivacaine 2mg/kg/min continued intravenous infusion to rat cardiac arrest; (7) D2: 0.9% saline 3ml/kg/min of continuous intravenous pump into five minutes, and then 0.5% ropivacaine 2mg/kg/min continuous intravenous infusion of 1 minute thoracotomy coring; (8) the E1 groups: 20% fat emulsion injection 3ml/kg/min continuous intravenous infusion 5 minutes, then 0.5% ropivacaine 2mg/kg/min continuous intravenous infusion to rats heartbeat arrest; (9) the E2 groups: 20% fat emulsion injection 3ml/kg/min continuous intravenous infusion 5 minutes, then 0.5% Ropivacaine 2mg/kg/min continuous intravenous infusion of 1 minute, thoracotomy coring ; continuous monitoring of mean arterial pressure and heart rate, record the rats before administration the basis of blood pressure (MAP), heart rate (HR) tracings normal electrocardiogram and blood pressure waveform, as a basis for reference and record the 1st room fatal rats appear arrhythmias (in QRS extended to 90 ms standard), cardiac arrest (electrocardiogram shows cardiac arrest was observed after 1 min ECG waveform no longer appear as a standard), and calculation of the corresponding phase of local anesthetic cumulative dose. Decapitation method for the control group and derived rats were sacrificed, the heart was quickly removed, frozen, ELISA method to measure ATP content. Results: 1. Reorganization between nine rats body weight difference was not statistically significant (P gt; 0.05). Hemodynamic baseline values ??the nine rats blood flow dynamics underlying value (MAP, HR) between the two groups was not statistically significant (P gt; 0.05) difference. The lethal group at each time point corresponding to the time and dose group C1, D1 group the E1 group rat ventricular arrhythmias (T2) and cardiac arrest (T3) than those in the B1 group delay, the difference was statistically significant ( P lt; 0.05), each corresponding phase, the amount of local anesthetic group C1, D1 group, E1 group than in the B1 group, the difference was statistically significant (P lt; 0.05). Group D1, E1 rats ventricular arrhythmias (T2) and cardiac arrest (T3) time than the C1 group delay, the difference was statistically significant (P lt; 0.05), each corresponding phase of local anesthetic dosage D1 Group, E1 group than C1 group and the difference was statistically significant (P lt; 0.05). Dosage of ropivacaine group D1 and E1 rats ventricular arrhythmias (T2) and heartbeat stop (T3), and each corresponding phase difference was not statistically significant (P gt; 0.05). 4.ATP content changes in ATP content comparison: A group gt; the group gt D2; the group gt C2; B2 group, any difference between the two groups were statistically significant (P lt; 0.05), D2, E2 group Inter was no significant difference (P gt; 0.05). Conclusions: intralipid can reduce cardiac toxicity of bupivacaine, the mechanism may be associated with increased myocardial ATP content, but no significant effect intralipid ropivacaine cardiotoxicity.

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