Dissertation > Medicine, health > Pharmacy > Pharmacology > Experimental Pharmacology

The Effects of Rosiglitazone and Prednisone on the Expression of FIZZ1 in the Pulmonary Fibrosis Induced by Blemycin

Author HuangQiuQin
Tutor XiaoQiMing
School Central South University
Course Department of Respiratory Medicine
Keywords Pulmonary fibrosis Bleomycin Prednisone Glucocorticoid Rosiglitazone Peroxisome proliferator-activated receptor γ Found in the inflammation area
CLC R965
Type Master's thesis
Year 2011
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Objective: To establish a model of bleomycin induced pulmonary fibrosis, dynamic expression and its significance the observation Fizzl in lung tissue, and comparing rosiglitazone with prednisone of Fizzl expression. Methods: 90 healthy male SD rats were randomly divided into normal control group (N), model group (B), prednisone intervention group (P group), Rogge rosiglitazone intervention group (R), a strong Song Luoge rosiglitazone intervention group (PR group) 5 group, n = 18 animals in each group at 7, 14, 28 days after modeling three time points were randomly divided into three subgroups, each subgroup only. Intratracheal injection of bleomycin (5mg/kg) one-time method to build a rat model of pulmonary fibrosis by intratracheal injection of the control group with normal saline as a control, making the intervention group was given the corresponding drugs die 24 hours after each irrigation. stomach. And modeling the corresponding point in time the animals were sacrificed, measured lung coefficient, lung tissue biopsy HE and Masson staining of pulmonary fibrosis, measured lung hydroxyproline (Hyp) content, detected by immunohistochemistry Fizzl expression in lung tissue. Results: 1 single intratracheal injection of bleomycin (5mg/kg) can be successfully constructed a rat model of pulmonary fibrosis. Lung coefficient of group B was significantly higher than the other groups (P lt; 0.01); P, R, PR group lung coefficient is lower than that in group B (P lt; 0.01), but higher than in group N (P lt; 0.05); between P, R, PR group compared to 7 days among the three groups was not statistically significant, 14 days PR group than in the P, R group (P lt; 0.05), 28-day PR group is lower than P group (P lt; 0.05). 3, the experimental group B and P, R, PR group at each time point in lung tissue HYP content has been higher than the N group (P lt; 0.01), with gradual increase; P, R, PR in each intervention group HYP content was low levels trend; P, R, PR group at all time points compared with group B decreased significantly (P lt; 0.01); 7 days P, R, RR group three was not statistically significant, 14 days, 28 days P, R group compared to the PR group was not statistically significant. , Blank control group alveolar structural integrity, no matrix thickening; the B group alveolitis and pulmonary fibrosis has been higher than in group N (P lt; 0.01), P, R, PR group at each time point in lung tissue biopsy destruction of the alveolar structure than in group B to reduce alveolitis and pulmonary fibrosis significantly reduced (P lt; 0.01), 14-day, 28-day PR group compared with the P group alveolitis and pulmonary fibrosis (P lt; 0.05), R group than in the PR group and P group than between the R group was not statistically significant (P gt; 0.05). 5, the experimental results of immunohistochemistry Fizzl expression is very weak in the N group, strong expression in the lung tissue of group B, is widely distributed in bronchial epithelial cells and alveolar type II epithelial cells, endothelial cells and macrophages, showed dynamic expression sex change. Seven days was strongest expression was decreased in 14 days, 28 days, but still stronger than the normal group (P lt; 0.05). P, R, PR each intervention rats Fizzl expression the Group B expression similar trend, but the expression level of each time point lower than that in group B (P lt; 0.05). 7 days, 14 days the rosiglitazone and prednisone combination therapy group than in the prednisone group Fizzl expression levels to reduce (P lt; 0.05), P group than in the R group, as well as the R group than in the PR group between no statistically significant (P gt; 0.05), 28 days P, R, PR group showed no statistical significance (P gt; 0.05) Conclusion: 1.Fizzl expression raised may be involved in bleomycin-induced pulmonary fibrosis formation. Rosiglitazone ketone, prednisone can be lowered Fizzl expression alleviate bleomycin-induced pulmonary fibrosis, reduce lung tissue collagen deposition prompt Fizzl may become a new target for treatment of pulmonary fibrosis, rosiglitazone The ketone and prednisone combined with treatment than single better with prednisone effect, the greater the reduction in levels Fizz1.

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