The Effect of Fentanyl on the Human Liver Cancinoma Cell Line HepG2 in Vitro
|School||Central South University|
|Keywords||Fentanyl HepG2 cells Cell proliferation Apoptosis|
Objective : To observe the fentanyl cultured human hepatoma cell line HepG2 cell proliferation activity , cell cycle and apoptosis . Methods : In vitro culture of human hepatoma cell line HepG2 , the experimental group in the culture medium with different concentrations of fentanyl (0.001,0.01,0.1,1,5,10,20 μmol / L), the control group without fentanyl, were cultured for 48 h , the the MTT colorimetric detection of human hepatoma cell line HepG2 proliferation activity , research fentanyl stimulate proliferation of HepG2 cells ; identified by Hoechst33258 analysis of fentanyl on apoptosis of HepG2 cells impact ; human hepatoma cell line HepG2 cell cycle by flow cytometry fentanyl . Results : 1 . Fentanyl concentrations ≥ 5μmol / L , the cell proliferation activity significantly lower than the control group ( P lt; 0.05 ) ; used by Hoechst33258 in the experimental group HepG2 cells apoptosis observed surface fluorescence microscope when fentanyl concentrations ≥ 0.1μmol / L , the fluorescence microscope to a lower proportion of apoptotic cells within the field of view of the total cell number increased significantly compared with the control group ( P lt; 0.01 ) ; With fentanyl concentration increased , The proportion of cells in G0/G1 phase was significantly higher ( S G2 / M ) phase cells was significantly lower . Conclusion: Fentanyl commonly used clinical dose range of human hepatoma cell line HepG2 cell proliferation and apoptosis of no significant impact , when fentanyl concentrations ≥ 0.1μmol / L can induce human hepatoma cell line HepG2 apoptosis concentrations ≥ 5μmol / L can inhibit the proliferation of human hepatoma cell line HepG2 .