Dissertation
Dissertation > Medicine, health > Pharmacy > Pharmacology > Experimental Pharmacology

The Effect of Fentanyl on the Human Liver Cancinoma Cell Line HepG2 in Vitro

Author SunZuoZuo
Tutor CaiHongWei
School Central South University
Course Anesthesiology
Keywords Fentanyl HepG2 cells Cell proliferation Apoptosis
CLC R965
Type Master's thesis
Year 2011
Downloads 22
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Objective : To observe the fentanyl cultured human hepatoma cell line HepG2 cell proliferation activity , cell cycle and apoptosis . Methods : In vitro culture of human hepatoma cell line HepG2 , the experimental group in the culture medium with different concentrations of fentanyl (0.001,0.01,0.1,1,5,10,20 μmol / L), the control group without fentanyl, were cultured for 48 h , the the MTT colorimetric detection of human hepatoma cell line HepG2 proliferation activity , research fentanyl stimulate proliferation of HepG2 cells ; identified by Hoechst33258 analysis of fentanyl on apoptosis of HepG2 cells impact ; human hepatoma cell line HepG2 cell cycle by flow cytometry fentanyl . Results : 1 . Fentanyl concentrations ≥ 5μmol / L , the cell proliferation activity significantly lower than the control group ( P lt; 0.05 ) ; used by Hoechst33258 in the experimental group HepG2 cells apoptosis observed surface fluorescence microscope when fentanyl concentrations ≥ 0.1μmol / L , the fluorescence microscope to a lower proportion of apoptotic cells within the field of view of the total cell number increased significantly compared with the control group ( P lt; 0.01 ) ; With fentanyl concentration increased , The proportion of cells in G0/G1 phase was significantly higher ( S G2 / M ) phase cells was significantly lower . Conclusion: Fentanyl commonly used clinical dose range of human hepatoma cell line HepG2 cell proliferation and apoptosis of no significant impact , when fentanyl concentrations ≥ 0.1μmol / L can induce human hepatoma cell line HepG2 apoptosis concentrations ≥ 5μmol / L can inhibit the proliferation of human hepatoma cell line HepG2 .

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