Dissertation
Dissertation > Medicine, health > Oncology > Genitourinary tumors > Male genitalia tumors > Prostate cancer

The Expression of miR-181b in Specimens of Prostate Cancer and the Effects of miR-181b on the Biological Behavior of Prostate Cancer PC-3 Cell Line

Author HeLong
Tutor QiuShi
School Liaoning Medical
Course Surgery
Keywords miR-181b prostate cancer PC-3 apoptosis proliferation invasion
CLC R737.25
Type Master's thesis
Year 2011
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Objective1. To study the expression of miR-181b in specimens of prostate cancer .2. To investigate the effects of miR-181b on the biological behavior of prostate cancer PC-3 cell line.Methods1. Tissues from 27 cases of prostate cancer and 30 samples of normal human prostate were collected after surgical operation.Total miRNA was extracted and the relative expression of miR-181b was quantified by Real-time PCR.2. miR-181b ASO was transfected into prostate cancer PC-3 cell.The expressions of miR-181b in transfected and non-transfected cells were measured by Real-time PCR;The changes of cell apoptosis were measured by flow cytometry;MTT assay and growth curve were used to assess the effect of miR-181b on cell proliferation;The changs of cell invasion abilities in vitro were detected by Transwell chamber.Results1. miR-181b was up-regulated in prostate cancer(P<0.05).2. Down-regulation of miR-181b may act as a potential genen therapy of prostate cancer by inducing apoptosis,inhibiting proliferation and depressing the invasion of PC-3 cells(P<0.05).ConclusionsmiR-181b was up-regulated in prostate cancer. Down-regulation of miR-181b may act as a potential genen therapy of prostate cancer by inducing apoptosis,inhibiting proliferation and depressing the invasion of PC-3 cells.

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