Expression of P27 kip1, Cyclin D1 and Skp2 in Basal Cell Carcinoma and Its Clinopathological Characters
|Keywords||Neoplasms, Basal Cell Cyclin-Dependent Kinase Inhibitor p27 S-Phase Kinase-Associated Proteins-2 Cyclin D1|
Objective: To investigate the expression of P27kip1, Cyclin D1, Skp2 and their relationship with the clinicopathologic features in basal cell carcinoma（BCC） . To explore their expression between with the subtypes of BCC of skin especially in morpheaform BCC subtype（IBCCs）.Methods: Twenty-six BCCs were divided into two groups（seven in morpheaform BCC subtype, IBCCs; nineteen in superficial and nodular BCC subtype, NIBCCs）. We used streptavidin-biotin peroxidase（SP） method of immunohistochemical staining for observing the expression of P27kip1, Cyclin D1 and Skp2 in 50 cases of BCC and 30 cases with normal tissues of skin in control group. The expression level was analyzed combined with clinicopathological features of age, gender and subtypes of tumor. Tissue sections were observed under optical microscope. All the data were conducted by spss13.0, chisquare test was used, P<0.05 was refered to statistically significant difference, P<0.01 was refered to statistically significant difference extremely. The relations of the three marks in BCC subtypes and IBCCs were conducted by Phi correlation analysis.Results:1.P27kip1 and Cyclin D1 positive products located in nucleus. Skp2 positive products located in cytoplasm or nucleus.2.The rate of positive expression of P27kip1 in BCC was 65.4%（17/26）, in normal skin tissues was 95.0%（19/20）（P<0.05）; P27kip1 in IBCCs was 28.6%（2/7）, in NIBCCs was 78.9% （15/19） （P<0.05）. 3.The rate of positive expression of Cyclin D1 in BCC was 38.5%（10/26）, in normal skin tissues was 5% （1/20）（P<0.05）; Cyclin D1 in IBCCs was 85.7%（6/7）, in NIBCCs was 21.2%（4/19） （P<0.05）.4.The rate of positive expression of Skp2 in BCC was 46.2%（12/26）, in normal skin tissues was 10% （2/20）（P<0.01）; Skp2 in IBCCs was 85.7%（6/7）, in NIBCCs was 31.6%（6/19） （P<0.05）.5.The rate of positive expression of P27kip1, Cyclin D1, Skp2 were no significant differences in age and sex of tumor（P>0.05）.6.Phi correlation analysis showed that: Cyclin D1 expression in BCC had negative correlation with P27kip1 expression. Correlation coefficient: r= -0.422,P<0.05;Skp2 expression in BCC had negative correlation with P27kip1 expression. Correlation coefficient: r= -0.624,P<0.05;Cyclin D1 expression in BCC had positive correlation with Skp2 expression. Correlation coefficient: r= 0.695,P<0.05.7. There were no correlated with each other of the three marks in IBCCs（P>0.05）.Conclusions:1.There is significant relationship between the location of P27kip1 and the grade of malignancy in certain human tumours.2.Low expression of P27kip1 and high expression of Cyclin D1 and Skp2 was in BCC, which indicate three of them may play an important role in carcinogenesis and the development of BCC. The expression of P27kip1 in IBCCs was lower than it in NIBCCs, but Cyclin D1 and Skp2 were reverse with P27kip1, which imply that they may play an important role in BCC subtypes.3.Cyclin D1 and Skp2 expression were negatively correlated with P27kip1, which indicates that Cyclin D1 and Skp2 are coordinating or co-regulating in the saturation and transference of tumor and promoting the occurrence and development of tumor together with P27kip1. Cyclin D1 expression was positively correlated with Skp2 in BCC, which indicates that there is reverse role in BCC.4. There were no significant differences in age and gender of tumor.And they are not associated with each other in IBCCs, due to fewer cases of this experiment can not be proven relation with IBCCs. We will keep working on it.This research investigats the roles of the regulation factors P27kip1, Cyclin D1 and Skp2 of cell cycle in the occurrence of BCC, and their relation with the clinopathological charaters and with the role in IBCCs, indicating that the examination of the three factors can be taken as important indicators for diagnosis and evaluation of the malignancy degree of BCC, and also make targets to treat it. It also reveals that the occurrence and development of BCC is due to the coordinated work or antagonism of many genes, and a joint test will help the accurate evaluation in the diagnosis and prognosis, can be useful factors to predict the subtypes of the tumor, also determine surgical margin for BCC cases.