The Association between cPLA2 Genetic Polymorphism and Alzheimer’s Disease in Hunan Han Population of China
|School||Central South University|
|Keywords||Alzheimer's disease Phospholipase A2 Single nucleotide polymorphisms|
Background and Objective: Alzheimer's disease (Alzheimer's disease, AD) is a kind of progressive cognitive impairment and memory deficits mainly degenerative disease of the central nervous system, which is characterized pathologically composed of p-amyloid senile plaques and to abnormal hyperphosphorylation of tau aggregation into a double helical filaments (paired helical filament, PHF) as the main component of neurofibrillary tangles. Its pathogenesis is unclear. Recent studies show that phospholipase A2 (phospholipase A 2, PLA2) mediated through p-amyloid (Ap) deposition, synaptic loss, oxidative stress and inflammatory reactions, the incidence of multiple links with AD closely in an important role in the occurrence of AD. Studies have found that cytosolic phospholipase A2 in Ap neuron toxicity. In recent years, foreign study found that cytosolic phospholipase A2 (cPLA2, PLA2G4A) gene polymorphism correlated with AD, suggesting that cPLA2 gene polymorphism in the pathogenesis of AD may play an important role. But the domestic has yet to see related literature. This study was designed through the analysis of cPLA2 PLA2G4A BanI, PLA2G4B BamHI two loci polymorphism with Chinese Han population in Hunan genetic susceptibility to Alzheimer's disease, to explore the genetics of Alzheimer's disease and the onset of the background mechanism for the diagnosis and treatment of AD genes provide a theoretical basis. Methods: In this study, case - control study, using polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) method to detect and analyze the southern Chinese Han population, 158 patients with AD and age, gender, matched healthy controls were 126 cases of cPLA2 PLA2G4A BanI, PLA2G4B BamHI two alleles, genotypes and their interactions. Results: 1. Chinese Han population in Hunan, PLA2G4A BanI locus genotype and allele frequencies were: Among AA.GA.GG genotype frequencies in the AD group were 49.4%, 38.6%, 12.0%, in in the control group were 53.2%, 36.5%, 10.3%, indicating that the genotype in the AD group and the control group had no significant difference in the distribution (P gt; 0.05). Allele A, G gene frequency distribution in the AD group were: 68.7%, 31.3% in the control group were 71.4%, 28.6%, indicating that the allele in the AD group and the control group, no significant distribution difference (0R = 1.141, P gt; 0.05). 2 Chinese Han population in Hunan, PLA2G4B BamHI genotype and allele frequencies were: Among AA, AG, GG genotype frequencies in the AD group were 56.3%, 34.2%, 9.5%, in the control group respectively, 18.3%, 42.1%, 39.7%, indicating that the AD group AA genotype was significantly higher, the difference was significant (P (0.05), prompted carry AA genotype had a higher risk of AD. etc. allele A, G gene frequency distribution in the AD group were: 73.4%, 26.6% in the control group were 39.3%, 60.7%, indicating that the AD group A allele frequency was significantly higher, the difference with significant (0R = 4.268, P <0.05). prompt individuals carrying the A allele may have a higher risk of AD. 3 in the \AG AA \\with the Chinese Han population in Hunan onset AD was no significant correlation. 2.PLA2G4B BamHI sites carrying the AA genotype or A allele may be associated with China Hunan Han population of AD pathogenesis. 3.PLA2G4A BanI locus and PLA2G4B BamHI polymorphic loci the risk of the presence of AD interaction.