Dissertation
Dissertation > Medicine, health > Pediatrics > Children within the science > Pediatric cardiac and vascular disease

The Relationship between Transforming Growth Factor Beta 1 Gene Polymorphism and Serum Concentration and Myocardial Fibrosis in Congenital Heart Disease

Author WangLanMei
Tutor XuYi
School Central South University
Course Pediatrics
Keywords Congenital heart disease Transforming growth factor β1 Gene polymorphism
CLC R725.4
Type Master's thesis
Year 2011
Downloads 57
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Objective To detect congenital heart disease (CHD) Children transforming growth factor β1 (TGFβ1) gene 869 polymorphism and serum concentrations of TGFβ1 , analyze TGFβ1 genotype , allele and serum concentrations investigate the relationship between TGFβ1 in congenital heart disease, myocardial fibrosis. Methods echocardiography confirmed the same period have congenital heart disease ( 20 cases of ventricular septal defect , patent ductus arteriosus, atrial septal defect in 7 cases and 14 cases ) , 25 males and 16 females . Age March -8.9 years old. Matched healthy children 33 cases , including 20 males and 13 females , aged May -7.1 years old. Fasting whole blood , plasma was separated and extracted mononuclear cell DNA, enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP), and serum concentrations were detected TGFβ1 the 869 polymorphism . Application SPSS16.0 statistical software to analyze serum concentrations of two groups of children , genotype, allele frequency distribution and the correlation between them . Results ① congenital heart disease group and the healthy control group of different genotypes meet Harding-Weinberg equilibrium ; ② group of children with congenital heart disease TGFβ1 serum concentrations than healthy control group difference was statistically significant (P lt; 0.01); ③ different type of group of children with congenital heart disease ( ventricular septal defect, patent ductus arteriosus and atrial septal defect ) showed no significant difference in serum concentrations (P gt; 0.05); ④ group of children with congenital heart disease compared with healthy control group genotype , allele frequency distribution difference was statistically significant (P lt; 0.05); ⑤ congenital heart disease group and the healthy control group , serum concentrations of different genotypes children TGFβ1 difference was statistically significant (P lt; 0.05), showing a TT, TC, CC increasing trend . Conclusion ① The serum concentrations of different genotypes , suggesting that TGFβ1 gene regulation by serum concentrations ; ② congenital heart disease group TGFβ1 ± 869CC genotype is susceptible genotype of myocardial fibrosis , congenital heart disease group carrying 869C allele children are more likely to myocardial fibrosis.

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