The Relationship between Transforming Growth Factor Beta 1 Gene Polymorphism and Serum Concentration and Myocardial Fibrosis in Congenital Heart Disease
|School||Central South University|
|Keywords||Congenital heart disease Transforming growth factor β1 Gene polymorphism|
Objective To detect congenital heart disease (CHD) Children transforming growth factor β1 (TGFβ1) gene 869 polymorphism and serum concentrations of TGFβ1 , analyze TGFβ1 genotype , allele and serum concentrations investigate the relationship between TGFβ1 in congenital heart disease, myocardial fibrosis. Methods echocardiography confirmed the same period have congenital heart disease ( 20 cases of ventricular septal defect , patent ductus arteriosus, atrial septal defect in 7 cases and 14 cases ) , 25 males and 16 females . Age March -8.9 years old. Matched healthy children 33 cases , including 20 males and 13 females , aged May -7.1 years old. Fasting whole blood , plasma was separated and extracted mononuclear cell DNA, enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP), and serum concentrations were detected TGFβ1 the 869 polymorphism . Application SPSS16.0 statistical software to analyze serum concentrations of two groups of children , genotype, allele frequency distribution and the correlation between them . Results ① congenital heart disease group and the healthy control group of different genotypes meet Harding-Weinberg equilibrium ; ② group of children with congenital heart disease TGFβ1 serum concentrations than healthy control group difference was statistically significant (P lt; 0.01); ③ different type of group of children with congenital heart disease ( ventricular septal defect, patent ductus arteriosus and atrial septal defect ) showed no significant difference in serum concentrations (P gt; 0.05); ④ group of children with congenital heart disease compared with healthy control group genotype , allele frequency distribution difference was statistically significant (P lt; 0.05); ⑤ congenital heart disease group and the healthy control group , serum concentrations of different genotypes children TGFβ1 difference was statistically significant (P lt; 0.05), showing a TT, TC, CC increasing trend . Conclusion ① The serum concentrations of different genotypes , suggesting that TGFβ1 gene regulation by serum concentrations ; ② congenital heart disease group TGFβ1 ± 869CC genotype is susceptible genotype of myocardial fibrosis , congenital heart disease group carrying 869C allele children are more likely to myocardial fibrosis.