Prostate Secretion Laboratory Inspection in Chronic Prostatitis Etiology Research
|School||Central South University|
|Keywords||Prostatitis Prostatic fluid Routine examination Etiology 16SrDNA|
Purpose by white blood cells in the prostate fluid routine check bacterial smear examination, prostatic fluid bacterial culture and sensitivity analysis, the prostate fluid the bacteria 16SrDNA gene detection of prostatic fluid of pathogenic microorganisms and chronic prostatitis relationship to further guidance chronic prostatitis clinical diagnosis and treatment. Method (1) observation of a semi-quantitative leukocyte count in the 218 specimens, comparing WBC ≥ 10 cells / field and the WBC lt; 10 / field situation, the detection rate of the bacterial culture and PCR methods, and mycoplasma, chlamydia detection rate; WBC ≥ 10 / vision of prostatic fluid specimens with Wright's dye staining in oil microscope leukocyte count, explore different types of white blood cells in patients with chronic prostatitis classification is different; prostatic fluid samples dye stained with Gram in oil microscope to find if there is a bacterial infection, the positive rates compared with both culture and PCR methods. (2) of the prostatic fluid bacterial culture and mycoplasma, chlamydia culture, and at the same time do susceptibility testing. Ordinary cultured using blood agar, Sabouraud petri dish, the L-type culture dish; Mycoplasma Ureaplasma people bidirectional selective medium, Chlamydia trachomatis by colloidal gold. (3) using the PCR method prostatic fluid the bacteria 16SrDNA gene, and compare the results with the culture test results. Results (1) using conventional methods leukocyte found in the two kinds of the identification method of the bacterial culture and PCR, the WBC LT; 10 / field and WBC ≥ 10 / the result of the field of vision is not a significant difference. Caused by bacterial infection, leukocytosis, elevated neutrophil. Prostatic fluid found bacterial smear, the bacterial smear positive rate lower than the bacterial culture and PCR methods. However, patients suitable for outpatient and urgent need to get the test results. (2) the common culture of the 218 cases of patients with prostatitis single bacteria infection 122 cases of mixed infection; L-type culture with a common culture-positive and pure L-type culture positive for the same kind of bacteria in 76 cases, three cases; Mycoplasma Chlamydia culture positive in 46 cases. Sensitivity to vancomycin in coagulase-negative staphylococci case sensitive only to vancomycin, rifampin, gentamicin, clindamycin, cotrimoxazole and other five kinds of drug sensitivity higher; Staphylococcus aureus, rifampin, gentamicin, chloramphenicol, doxycycline sensitivity; Escherichia coli amikacin, fosfomycin, meropenem, piperacillin, cephalosporins (cefoperazone, cefotaxime, ceftazidime, cefuroxime), fluoroquinolones (including ciprofloxacin, levofloxacin), nitrofurantoin due sensitivity. (3) polymerase chain reaction (Polymerase chain reaction, PCR) to detect prostatic fluid of patients with chronic prostatitis bacterial 16SrDNA gene, 218 specimens positive bacterial culture in 124 cases, 124 cases of culture-positive specimens the PCR was all positive, all bacterial prostatitis; 93 specimens culture negative, PCR, the positive rate of 84.9%. Bacterial culture and PCR assay results comparing the PCR method was higher than culture. Conclusion (1) the white blood cells of patients with type II prostatitis does not necessarily appear elevated, there prostatitis symptoms, even if the white blood cells in the normal also suggested a pathogenic examination, so as not to be mistaken for type Ⅲ B prostatitis. (2) The bacterial prostatitis patients, such as the white blood cell count, mainly Zeyi neutrophils increased. (3) bacterial smear apply to outpatient and urgent need to make the diagnosis of patients. (4) bacterial infection is one of the main cause of prostatitis, Gram-negative bacteria Escherichia coli, Klebsiella, Gram-positive bacteria Staphylococcus aureus and coagulase-negative grape cocci. Mycoplasma, chlamydia infection is one of the main cause of prostatitis. (5) 16SrDNA gene detection can increase the rate of diagnosis of bacterial prostatitis.