Inflammatory and coagulation factors and CRP1059G / C gene polymorphism and pulmonary thromboembolism associated
|School||Xinjiang Medical University|
|Keywords||Pulmonary thromboembolism High-sensitivity C -reactive protein Gene polymorphism D-dimer Coagulation factor Ⅶ|
Objective: To evaluate pulmonary thromboembolism (PTE) in patients with serum high-sensitivity C-reactive protein (Hs-CRP) levels, plasma D-dimer (DD) levels, blood coagulation factor Ⅶ levels (F Ⅶ a) and CRP 1059 G / C polymorphism Normality and pulmonary thromboembolism (PTE) correlation. Methods: Case selected from the First Affiliated Hospital of Xinjiang Medical University of PTE patients 213 cases, 213 cases of the control group over the same period in our hospital volunteers. Cases against 164 cases and 82 cases of group control group using enzyme-linked immunosorbent assay (ELESA) of serum Hs-CRP and F Ⅶ a concentration, while the above-mentioned cases detected by immune nephelometry plasma DD levels. Polymerase chain reaction - restriction fragment length polymorphism (polymerase chain reaction restriction fragment length polymorphism, PCR-RFLP) typing technique detects nucleotide CRP1059G / C polymorphism loci, according to gel electrophoresis bands seen in the study distinguish between genotype and genotype frequency statistics. Results: 1) PTE serum Hs-CRP levels (35.79 ± 3.12 pg / ml) was significantly higher (10.03 ± 1.23pg/ml) (P = 0.028); 2) PTE DD plasma levels (1359.47 ± 337.04 ) g / L, the control group, plasma DD levels (360.00 ± 90.5) g / L, respectively, the difference was statistically significant (P lt; 0.05); 3) PTE F Ⅶ a serum level (209.80 ± 87.89) pg / ml was significantly higher serum F Ⅶ a level (71.27 ± 26.83) pgml, (P lt; 0.05); 4) GG genotype in the case group accounted for 88.8%, GC type accounted for 7.5%, CC type accounted for 3.7% in the control group and the GG genotype accounted for 86.4%, GC type accounted for 10.3%, CC type accounted for 3.3%, the overall G allele frequency was 91.5%, the overall C allele frequency was 8.5%; 5) PTE group and control group Compare CRP 1059 G / C genotypes and G, C allele frequency was no significant difference between the two groups; 6) Multivariate Logistic regression analysis showed that: diabetes, venous disease history, PTE history, fibrinogen, white blood cell count, Hs-CRP, DD, F Ⅶ a PTE is an independent risk, after adjustment for these risk factors, they found Hs-CRP, DD, F Ⅶ a PTE is still a risk factor. Conclusion: inflammation may be involved in the pathological process of pulmonary thromboembolism, PTE pathological processes in blood coagulation and fibrinolysis in a high state, and the extrinsic coagulation mechanisms may be involved in the formation of pulmonary thromboembolism pathological process. Case group CRP 1059 G / C genotypes and allele frequencies compared with the control group with no significant difference. Hs-CRP, DD, F Ⅶ a risk factor for elevated PTE.