Dissertation > Agricultural Sciences > Crop > Cereal crops > Rice

The Study of Regulation of ROS Levels in Rice Seedling by SOD Model Compounds

Author ChenZuoZhi
Tutor LiuChangLin
School Central China Normal University
Course Inorganic Chemistry
Keywords Reactive oxygen species SOD model compounds UV - visible spectroscopy Histochemical method Confocal laser scanning microscope
CLC S511
Type Master's thesis
Year 2009
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Reactive oxygen species (reactive oxygen species, ROS) has been considered is a byproduct produced by the aerobic metabolism of plants , and may be anti - oxidant and antioxidant enzymes Clear . More recently, however, it was discovered that reactive oxygen species as signal molecules , play an important role in the process , such as plant growth , development, hormone signaling , response to biotic or abiotic environmental stress and programmed cell death . In this paper , a model compound of copper-zinc superoxide dismutase ( SOD mimetic enzyme ) soaked rice seeds under drought stress , the use of UV - visible spectroscopy , fluorescence spectroscopy , histochemistry , laser scanning confocal microscopy in vivo activity of rice the variation of the oxygen level . UV - visible spectroscopy analysis of the rice body's antioxidant enzyme activity levels change . First, using UV - visible spectroscopy , fluorescence spectroscopy rice plants H 2 O 2 O 2 < / sub > - < / sup · OH content of variation test , and found that the treatment groups were lower than the corresponding control group . Second, dimethyl benzidine (DAB) and nitro blue tetrazolium ( the nitroblue tetrazolium NBT ) histochemical staining method in rice leaves H 2 O 2 < / sub > O 2 - < / sup > levels in situ monitoring , found SOD simulate enzyme treatment to reduce the rice leaves under drought stress H < / sub> O 2 O 2 - accumulation . Third, the use of a fluorescent dye H 2 DCFDA and fluorescence microscopy and confocal laser microscopy rice root H 2 O 2 changes in the level imaging analysis found that SOD analog enzyme - treated rice roots H 2 O 2 content , lower than the corresponding control group H 2 O 2 < / sub> content . Fourth, the use of UV - visible spectroscopy rice leaf extract antioxidant enzyme activity in the liquid is determined . The study found that under drought stress conditions in the treatment group four antioxidant enzymes : SOD , CAT ( catalase ) , APX ( ascorbate peroxidase ) and GR ( glutathione reductase ) activity are high in the control group .

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