Association of Genetic Polymorphism in Brain Natriuretic Peptide with Essential Hypertension in Hunan Han
|School||Central South University|
|Keywords||Brain natriuretic peptide Essential hypertension Single nucleotide polymorphisms|
Background: Hypertension (Essential Hypertension, EH) is one of the most common cardiovascular disease is a major public health problem worldwide. Essential hypertension is a complex disease caused by genetic factors and environmental factors. 30-60% of the population blood pressure variability is due to genetic factors. Hypertension genetic risk factors, they use a strategy of candidate genes, genes in the brain natriuretic peptide (BNP)] chromosome the p36.2 position on one of the candidate genes for hypertension genetic susceptibility. BNP is a peptide hormone of a cardiovascular, can activate the natriuretic peptide receptor A bird cyclase, leading to cGMP production and activation of downstream signaling cascades, thereby generating a series of changes in biological activity of the target tissue, including diastolic arteriovenous promote sodium excretion, and inhibition of myocardial fibrosis and hypertrophy, which involved in the pathophysiology of hypertension process. Abroad BNP rs 198388 (G / A) gene polymorphism with essential hypertension susceptibility reported, but China has yet to see similar reports. Objective: To investigate brain natriuretic peptide (BNP) rs198388 gene polymorphism and Hunan Han population with essential hypertension genetic susceptibility relationship. Methods: Polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) analysis method, the BNP gene rs198388 sites were genotyped in 567 cases of patients with essential hypertension and 555 healthy population. The application of the similarities and differences of the distribution of genotypes and A allele frequencies χ2 test was used to compare the groups, according to the genotype and A allele frequency distribution of the similarities and differences in the gender-stratified analysis in the case and control groups; application of non-conditional logistic regression analysis of risk factors correction to determine the correlation between BNP rs198388 genotype with essential hypertension in genetic susceptibility to ask; among different genotypes general differences in the application of single-factor analysis of variance comparing hypertensive and control groups. Results: 1. Cases of hypertension group BNP gene rs198388 polymorphic loci GG, GA and AA genotype frequencies were 87.7%, 10.9% and 1.4%, control group rs198388 polymorphic loci GG, GA and AA genotype frequencies were 81.6%, 17.3% and 1.1%, genotype distribution were in Hardy-Weinberg equilibrium; genotype distribution between cases and controls there was a significant difference (χ2 = 9.513, P = 0.009); cases of hypertension group rs198388 The allele frequency of 6.9%, the control group was 9.7%, the the rs198388 allele frequency of hypertension cases and controls in the distribution of a statistically significant difference (χ2 = 6.000, P = 0.014); Logistic regression analysis BNP rs198388 polymorphism with essential hypertension risk carrying rs198388 A bit lower risk of individuals suffering from essential hypertension (OR = 0.344,95% CI, :0.187-0 .633, P = 0.0006 ). Lower risk Conclusion: BNP rs198388 of genetic polymorphism may be associated with the susceptibility of essential hypertension in Hunan Han population and carry rs198388 A allele individuals suffering from essential hypertension.