Dissertation > Medicine, health > Internal Medicine > Heart, blood vessels ( circulatory ) disease > Heart disease > Coronary arteries ( atherosclerosis ),heart disease (CHD)

PECAM-1 Gene Polymorphisms in the Exons 3 and 8 with Coronary Heart Disease in the Han of Western Liaoning

Author HuangLiFeng
Tutor ZhangYunLi
School Liaoning Medical
Course Clinical Laboratory Science
Keywords Platelet endothelial cell adhesion molecule-1 Coronary heart disease Gene polymorphism
CLC R541.4
Type Master's thesis
Year 2011
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Objective 1 study platelet endothelial cell adhesion molecule -1 (Platelet-endothelial cell adhesion molecule-1, PECAM-1) in the Han population of western Liaoning Province exon 3 sub-G373C and exon the A1688G two gene polymorphism and coronary heart disease, and to explore the relationship PECAM-1 gene G373C and A1688G single nucleotide polymorphism and coronary artery stenosis degree of population with coronary heart disease. Plasma soluble PCAM-1 level of relations with the Han population of coronary heart disease in the west of Liaoning province. Methods in western Liaoning Han 153 patients with coronary heart disease and 200 healthy controls, peripheral blood genomic DNA was extracted using the the Takara DNA blood extraction kit using the polymerase chain reaction - restriction fragment length polymorphism (polymerase chain reaction-restriction fragment length polymorphism, PCR-FRLP) exon G373C PECAM-1 Exon 3 and 8 outside the exon the A1688G conducted genotyping. In this paper, SPSS software to analyze the experimental results with Logistic regression analysis of the relationship between genotypes and coronary heart disease; PECAM-1 concentration in the plasma of patients and controls was measured by enzyme-linked immunoassay (ELISA), with biochemical techniques detect lipid levels. 1, PECAM-1 exons G373C 3 and 8 outside exon the A1688G two-locus genotype and allele distribution frequency distribution in coronary heart disease patients and healthy control group, there were significant differences. Distribution of coronary heart disease (Coronary heart disease, CHD) group of coronary artery stenosis in patients with PECAM-1 G373C gene studies have shown that portable GC and GG genotype in patients with a relative risk of coronary artery severe stenosis carrying the CC genotype, 2.49 times and 2.95 times. CHD group, patients with coronary stenosis PECAM-1 A1688G gene distribution difference was not statistically significant. 4 CHD group compared with control group, PECAM-1 serum levels have significant differences. PECAM-1 G373C and A1688G genotype and serological level in the CHD group showed significant differences. Conclusion 1 (PECAM-1) gene 373G / C and 1688A / G gene polymorphism and western Liaoning Province Han coronary heart disease correlation. PECAM-1 G373C gene polymorphism genotypes and coronary artery stenosis degree distribution of a statistically significant difference, portable GC and GG genotype may increase the risk of severe coronary artery stenosis. 3, PECAM-1 G373C, A1688G two points gene polymorphism can affect PECAM-1 serum levels.

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