Dissertation
Dissertation > Medicine, health > Clinical > Therapy > Diet,Journal of Clinical Nutrition

The Influence of Aging and Enteral Nutrition on Glucose and Lipid Metabolism and Intestinal Mucosa Epithelial Barrier in Rats

Author LiXi
Tutor HuYu
School Fudan University
Course Internal Medicine
Keywords Increasing age Glucose and lipid metabolism Claudin Enteral Nutrition Protection
CLC R459.3
Type Master's thesis
Year 2011
Downloads 38
Quotes 0
Download Dissertation

Background and purpose: the elderly normal aging in the body on the structure and function of the process of the emergence of various degenerative changes, metabolic disorders and intestinal epithelial barrier injury incidence gradually increased. The effect of enteral nutrition in the treatment of acute intestinal mucosal barrier injury significantly, but on glucose and lipid metabolism in the elderly and in the treatment of aging-related intestinal mucosal barrier of the injured party and also a lack of evidence. SD rats, this study through the different months (1) understanding of aging on glucose and lipid metabolism in normal healthy SD and enteral nutrition on glucose and lipid metabolism in elderly SD, enteral nutrition in older SD The rats used in security evaluation: (2) to understand the increasing age of the intestinal epithelial barrier of normal healthy SD rats, and enteral nutrition intervention whether the protective effect of the changes in the elderly SD rat intestinal mucosal barrier. Materials and methods: (1) select 20 3-month-old SD rats were randomly divided into 3-month-old conventional diet group (A) and 3-month-old conventional diet and enteral nutrition feeding group (C), select 20 12 old SD rats were randomly divided into 12-month-old conventional diet group (B), and 12-month-old conventional diet and enteral nutrition feeding group (D). Conventional feeding and drinking feeding conventional diet group in January, the conventional diet and enteral nutrition feeding group intragastric administration of enteral nutrition in conventional feeding and drinking on the basis of agent (100 Standard), 2 times / day, a total of last January. Measured every 10 days of the feeding period the rats weight change, feeding rats biochemical indicators (TG, TC, LDL-C, HDL-C, FBG, FINS, ALT, AST, ALB, TBIL) detected after January HOMA model insulin resistance index (HOMA-IR) (2) Select the 20 3-month-old SD rats were randomly divided into 3-month-old conventional diet group (A) and 3-month-old conventional diet and enteral nutrition feeding group (C group), select 20 to 12-month-old SD rats were randomly divided into two 12-month-old conventional diet group (B), and 12-month-old conventional diet and enteral nutrition feeding group (D). Conventional feeding and drinking feeding conventional diet group in January, the conventional diet and enteral nutrition feeding group intragastric administration of enteral nutrition in conventional feeding and drinking on the basis of agent (100 Standard), 2 times / day, a total of last January. Rearing January after rat liver, spleen, mesenteric lymph node tissue bacterial culture to calculate the rate of bacterial translocation; take the rat ileum conventional HE staining morphological changes, the use of semi-quantitative RT-PCR technology Occludin and ZO-1 mRNA expression levels in the small intestinal mucosa specimens were analyzed; using immunohistochemistry to detect the small intestinal mucosa specimens Occludin and ZO-1 protein levels. (3) compare the 3-month-old conventional diet group (A) and 12-month-old conventional diet group (B), 3-month-old and enteral nutrition group (C) and 12-month-old joint enteral nutrition group (D ), 3-month-old conventional diet group (A) and enteral nutrition group (C), 12-month-old conventional diet group (B) and enteral nutrition group (D) between the weight and biochemical parameters (TG, TC, LDL-C, HDL-C, FBG, FINS, ALT, AST, ALB, TBIL), HOMA insulin resistance index (HOMA-IR) difference model. (4) comparing the 3-month-old conventional diet group (A) and 12-month-old conventional diet group (B), 3-month-old conventional diet group (A) and enteral nutrition group (C), 12-month-old conventional diet group (B) and enteral nutrition group (D) between the small intestinal mucosa specimens Occludin and ZO-1 mRNA expression levels Occludin and ZO-1 protein levels, intestinal bacterial translocation rate differences. Results: (1) conventional diet for January, 12-month-old rats (B group), compared with the 3-month-old rats (A group): slower weight gain (P lt; 0.05); TG, TC, LDL -C levels (P lt; 0.05), HDL-C levels decreased (P lt; 0.05), FINS levels (P lt; 0.05), more than biochemical indicators (FBG, ALT, AST, ALB, TBIL) significant differences, HOMA-IR increased (P lt; 0.05). Conventional diet and enteral nutrition feeding January, 12-month-old rats (D group) and 3-month-old rats (C group) compared: slower weight gain (P lt; 0.05); TG, TC, LDL- C levels (P lt; 0.05), HDL-C levels decreased (P lt; 0.05), FINS levels (P lt; 0.05), more than biochemical indicators (FBG, ALT, AST, ALB, TBIL) no significant differences, HOMA-IR increased (P lt; 0.05). Feeding after January, the 3-month-old conventional diet joint enteral nutrition group rats (C group) compared with the same month-old conventional diet group rats (A group), weight gain (P lt; 0.05), biochemical parameters (TG TC, LDL-C, HDL-C, FBG, FINS, ALT, AST, ALB, TBIL) no significant differences, HOMA-IR was no significant difference. Feeding after January, the 12-month-old conventional diet joint enteral nutrition group rats (D group) compared with the same month-old conventional diet group rats (B group), weight gain (P lt; 0.05), biochemical parameters (TG TC, LDL-C, HDL-C, FBG, FINS, ALT, AST, ALB, TBIL) no significant differences, HOMA-IR was no significant difference. (2) conventional diet for 1 month after the 12-month-old rats (B) compared with the 3-month-old rats (A group): intestinal mucosal thickness and villus height reduction (P lt; 0.05), the small intestinal mucosa tight junction protein mRNA (Occludin mRNA, ZO-1 mRNA) (P lt; 0.05), decreased expression of the small intestine mucosa tight junction protein (Occludin, ZO-1) reduce (P lt; 0.05), the rate of bacterial translocation There was no significant difference. The rearing January, 3 months old conventional diet combined enteral nutrition rats (C group) compared with the same month-old conventional diet group rats (A group), intestinal mucosal thickness and villus height was no significant difference in the small intestinal mucosa connexin mRNA (of Occludin mRNA, ZO-1 mRNA in) no significant difference in expression closely, the small intestinal mucosa tight junction protein (of Occludin, ZO-1) no significant difference was no significant difference in the rate of bacterial translocation. Rearing January, the 12-month-old conventional diet combined enteral nutrition rats (D group) compared with the same month-old conventional diet group rats (B group), intestinal mucosal thickness and villus height increased (P lt; 0.05), tight junction protein mRNA (Occludin mRNA, ZO-1 mRNA) in the small intestinal mucosa (P lt; 0.05), increased expression of the small intestine mucosa tight junction protein (Occludin, ZO-1) increased (P lt; 0.05), intestinal was no significant difference in the rate of bacterial translocation. Conclusion: (1) grow older, aged SD rats and young SD rats compared: weight gain slows down: TG, TC, LDL-C levels, reduced levels of HDL-C, FINS level rise, I biochemical indicators (FBG, ALT, AST, ALB, TBIL) no significant difference, HOMA-IR increased. , Jan. feeding and enteral nutrition in elderly SD rats compared with the conventional diet aged SD rats, weight gain, biochemical parameters (TG, TC, LDL-C, HDL-C, FBG, FINS, ALT, AST , ALB, TBIL) no significant difference, HOMA-IR was no significant difference. Enteral Nutrition (100 Standard) elderly SD no significant effect on glucose and lipid metabolism in older SD rats can be used safely. (2) increase with age, older SD rats compared with young SD rats, intestinal mucosal thickness and villus height decreased, decreased expression of tight junction protein mRNA (Occludin mRNA, ZO-1 mRNA) in the small intestinal mucosa, small intestine Claudin mucosal tissues (Occludin, ZO-1) reduced bacterial translocation rate was no significant difference. Feeding after January, and enteral nutrition in elderly SD rats compared with the conventional diet aged SD rats, intestinal mucosal thickness and villus height increased Claudin small intestinal mucosa the nRNA (of Occludin mRNA, ZO-1 mRNA) Increased expression of tight junction protein (Occludin, ZO-1) increased in the small intestinal mucosa, bacterial translocation rate was no significant difference. Reduce the degree of tight junction proteins in rat intestinal epithelial barrier Enteral Nutrition (100 Standard) can improve older SD (Occludin, ZO-1) on the the aging intestinal mucosa barrier injury has a protective effect.

Related Dissertations
More Dissertations