Immunoglobulin G Expression in Hepatocytes of Adult Rat Liver
|Course||Pathology and Pathophysiology|
|Keywords||Hepatocytes Immunoglobulin G Albumin Alpha-fetoprotein Microdissection Expression|
The background and purpose of the classical theory , immunoglobulin G ( immunoglobulin G ) is only synthesized by the differentiation and maturation of B lymphocytes . In recent years, more and more researchers showed that malignancies of epithelial origin and in vitro cultured cell lines , such as liver cancer , breast cancer , etc. , can also produce and secrete immunoglobulin G . Meanwhile, many researchers also found that , in normal cells , the expression of immunoglobulin G . Immunoglobulin G is expressed in mature liver cells has not been elucidated . This paper studies in SD rats mature liver cells , the expression of immunoglobulin G . Materials and Methods 1. Study 10 patients weighing 250 - 300g male adult Sprague-Dawley rat liver tissue . The rat strains purchase Experimental Animal Center of Shantou University Medical College . 2 immunohistochemistry ( Immunohistochemistry IHC ) , immunoglobulin G protein detection paraffin tissue sections . In situ hybridization ( in situ hybridization , ISH ) detection paraffin tissue sections immunoglobulin G mRNA expression . Microdissection single - cell RT-PCR technology (laser capture microdissection (LCM) related single-cell RT-PCR) detection of immunoglobulin G mRNA expression in fresh tissue . 1 immunohistochemistry in paraffin sections of adult rat liver tissue , liver cell cytoplasm staining of immunoglobulin G signal . (2) in situ hybridization in paraffin sections of adult rat liver tissue , liver cell cytoplasm immunoglobulin G mRNA expression . Immunoglobulin G mRNA expression in adult rat liver cells . Microdissection single cell PCR technology microdissection extracted . Conclusion , immunoglobulin G protein and mRNA expression in the adult rat liver cells .