Dissertation
Dissertation > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology

Matrine injection on the treatment of acute radiation enteritis and its mechanism

Author ChenYanQun
Tutor LiangQingMo
School Nanhua University
Course Oncology
Keywords Traditional Chinese medicine Matrine injection Radiation enteritis Radiological Protection NO
CLC R285
Type Master's thesis
Year 2011
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Objective: To investigate matrine injection of radiation-induced acute radiation enteritis protective effect and its mechanism for the clinical application of matrine injection prevention of acute radiation enteritis provide theoretical and experimental basis. Methods: The subjects selected 42 male SD rats were randomly divided into four groups, Ⅰ group of normal control group (n = 6), Ⅱ group experimental control group (n = 12), Ⅲ group matrine injection high irradiation dose group (n = 12), Ⅳ group matrine injection irradiated low-dose group (n = 12). Experimental day 1, will Ⅱ, Ⅲ, Ⅳ rats with 10% chloral hydrate anesthesia after intraperitoneal injection of 400mg/kg, 6MV herein are the X-ray 10Gy, source skin distance 100cm whole abdomen single irradiation to produce large rat model of acute radiation enteritis. 8 hours after irradiation of rats by intraperitoneal injection: Ⅰ, Ⅱ group were given normal saline 2ml; Ⅲ group I matrine injection of high doses of saline (containing liquid 2ml/kg); Ⅳ group I low-dose matrine injection saline (containing liquid 0.4ml/kg). Intraperitoneal injection for 7 days, once a day. Rats after irradiation general and stool and other changes. Rats were sacrificed on day 8, the rats weighing scales to record weight values. Take 3cm ileum with 10% formalin-fixed, paraffin-embedded, HE staining, rats were observed under light microscope morphological changes of the ileum, counting the number of villi / cm, calculate the average villus height. Another 5cm long terminal ileum, ground into a 10% homogenates, 4000R/min after 10min take 0.5ml centrifuge supernatant, using nitrate reductase assay ileal tissue NO levels. Results: 1, the rats generally: the rats were alive, Ⅰ group were good appetite, responsive, and body hair smooth and shiny. Group Ⅱ listlessness, loss of appetite significantly decreased, unresponsive, body hair obscure. Ⅲ, Ⅳ rats after irradiation appetite has decreased, three days after the spirit, appetite improved, the reaction is more sensitive body hair smooth. Ⅲ, Ⅳ rats showed no other adverse reactions. 2, the rats were observed after irradiation daily stool: normal control rats daily stool shape, granular. Exposed on the third day Ⅱ, Ⅲ, Ⅳ group were diarrhea in rats, showed mucoid stools, watery stools, containing undigested food, and so on. Group Ⅱ duration of diarrhea and diarrhea are more than the number of rats Ⅲ, Ⅳ groups. 3, the body weight of rats in each group: experiments on the 8th day, Ⅰ rats increased body weight, was (276.83 ± 18.68g), Ⅱ rats body weight (210.33 ± 21.71g), Ⅲ rats body weight (269.33 ± 17.70 g), Ⅳ rats body weight (254.50 ± 14.87g). Weight gain in rats Ⅲ group Ⅰ basically the same. Experimental Section 8 days, Ⅱ rats weighing falling instead of rising, Ⅱ rats body weight compared with group Ⅰ were significantly different (P lt; 0.001), while the body weight of rats in group Ⅲ and group Ⅰ was no significant difference (P gt; 0.05). And body weight of rats in group Ⅳ Ⅰ, Ⅲ group contrast differences (P lt; 0.05). 4, each rat ileum morphological changes, counting and measuring the average number of villi villus height: a) each rat ileum morphological changes: Ⅰ rats villi slender, structured, there is a certain height, crypt deep; Ⅱ group villi low, sparse, loss, edema, crypt shallow, obvious inflammatory cell infiltration, wall thinning; Ⅲ group ileum histological manifestations and is basically the same group Ⅰ; Ⅳ group shorter villi , crypt depth than group Ⅰ shallow, some inflammatory cell infiltration. 2) the rats villi count: Ⅰ group was 77 ± 8 个 / cm, Ⅱ group was 51 ± 8 个 / cm, Ⅲ group was 74 ± 10 个 / cm, Ⅳ group was 67 ± 4 个 / cm. 3) rats in each group average ileum villus height: Ⅰ group was 287.20 ± 30.87μm, Ⅱ group was 196.24 ± 27.64μm, Ⅲ group was 283.88 ± 47.17μm, Ⅳ group was 260.31 ± 38.59μm. Ⅱ group number and the villi villus height and Ⅰ, Ⅲ, Ⅳ group were significantly different (P lt; 0.001), Ⅲ Ⅰ group rats compared with the above two parameters were not significantly different (P gt; 0.05) . 5, matrine injection on rat ileal mucosa NO Level: ileal mucosa of rats in each group NO concentration: Ⅰ group was 0.25 ± 0.03μmol / g; Ⅱ group was 0.55 ± 0.09μmol / g; Ⅲ group was 0.26 ± 0.02μmol / g; Ⅳ group was 0.28 ± 0.06μmol / g. Group Ⅱ ileal mucosa NO levels were significantly higher than Ⅰ, Ⅲ, Ⅳ group (P lt; 0.001), Ⅳ group NO levels higher than group Ⅰ (P lt; 0.05), Ⅲ group was close to the level of group Ⅰ NO NO levels, no significant difference (P gt; 0.05), Ⅲ and Ⅳ were compared between group differences (P lt; 0.05). Conclusions: 1. Matrine injections can relieve acute radiation enteritis gastrointestinal reactions and pathological changes, suggesting that matrine injection on acute radiation enteritis have a better therapeutic effect. 2 matrine injection can reduce the concentration of NO in the small intestinal mucosa, matrine injection treatment of acute radiation enteritis mechanism of action may be relevant. 3 matrine injection of high doses better than the low-dose group, no adverse reactions.

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