Matrine injection on the treatment of acute radiation enteritis and its mechanism
|Keywords||Traditional Chinese medicine Matrine injection Radiation enteritis Radiological Protection NO|
Objective: To investigate matrine injection of radiation-induced acute radiation enteritis protective effect and its mechanism for the clinical application of matrine injection prevention of acute radiation enteritis provide theoretical and experimental basis. Methods: The subjects selected 42 male SD rats were randomly divided into four groups, Ⅰ group of normal control group (n = 6), Ⅱ group experimental control group (n = 12), Ⅲ group matrine injection high irradiation dose group (n = 12), Ⅳ group matrine injection irradiated low-dose group (n = 12). Experimental day 1, will Ⅱ, Ⅲ, Ⅳ rats with 10% chloral hydrate anesthesia after intraperitoneal injection of 400mg/kg, 6MV herein are the X-ray 10Gy, source skin distance 100cm whole abdomen single irradiation to produce large rat model of acute radiation enteritis. 8 hours after irradiation of rats by intraperitoneal injection: Ⅰ, Ⅱ group were given normal saline 2ml; Ⅲ group I matrine injection of high doses of saline (containing liquid 2ml/kg); Ⅳ group I low-dose matrine injection saline (containing liquid 0.4ml/kg). Intraperitoneal injection for 7 days, once a day. Rats after irradiation general and stool and other changes. Rats were sacrificed on day 8, the rats weighing scales to record weight values. Take 3cm ileum with 10% formalin-fixed, paraffin-embedded, HE staining, rats were observed under light microscope morphological changes of the ileum, counting the number of villi / cm, calculate the average villus height. Another 5cm long terminal ileum, ground into a 10% homogenates, 4000R/min after 10min take 0.5ml centrifuge supernatant, using nitrate reductase assay ileal tissue NO levels. Results: 1, the rats generally: the rats were alive, Ⅰ group were good appetite, responsive, and body hair smooth and shiny. Group Ⅱ listlessness, loss of appetite significantly decreased, unresponsive, body hair obscure. Ⅲ, Ⅳ rats after irradiation appetite has decreased, three days after the spirit, appetite improved, the reaction is more sensitive body hair smooth. Ⅲ, Ⅳ rats showed no other adverse reactions. 2, the rats were observed after irradiation daily stool: normal control rats daily stool shape, granular. Exposed on the third day Ⅱ, Ⅲ, Ⅳ group were diarrhea in rats, showed mucoid stools, watery stools, containing undigested food, and so on. Group Ⅱ duration of diarrhea and diarrhea are more than the number of rats Ⅲ, Ⅳ groups. 3, the body weight of rats in each group: experiments on the 8th day, Ⅰ rats increased body weight, was (276.83 ± 18.68g), Ⅱ rats body weight (210.33 ± 21.71g), Ⅲ rats body weight (269.33 ± 17.70 g), Ⅳ rats body weight (254.50 ± 14.87g). Weight gain in rats Ⅲ group Ⅰ basically the same. Experimental Section 8 days, Ⅱ rats weighing falling instead of rising, Ⅱ rats body weight compared with group Ⅰ were significantly different (P lt; 0.001), while the body weight of rats in group Ⅲ and group Ⅰ was no significant difference (P gt; 0.05). And body weight of rats in group Ⅳ Ⅰ, Ⅲ group contrast differences (P lt; 0.05). 4, each rat ileum morphological changes, counting and measuring the average number of villi villus height: a) each rat ileum morphological changes: Ⅰ rats villi slender, structured, there is a certain height, crypt deep; Ⅱ group villi low, sparse, loss, edema, crypt shallow, obvious inflammatory cell infiltration, wall thinning; Ⅲ group ileum histological manifestations and is basically the same group Ⅰ; Ⅳ group shorter villi , crypt depth than group Ⅰ shallow, some inflammatory cell infiltration. 2) the rats villi count: Ⅰ group was 77 ± 8 个 / cm, Ⅱ group was 51 ± 8 个 / cm, Ⅲ group was 74 ± 10 个 / cm, Ⅳ group was 67 ± 4 个 / cm. 3) rats in each group average ileum villus height: Ⅰ group was 287.20 ± 30.87μm, Ⅱ group was 196.24 ± 27.64μm, Ⅲ group was 283.88 ± 47.17μm, Ⅳ group was 260.31 ± 38.59μm. Ⅱ group number and the villi villus height and Ⅰ, Ⅲ, Ⅳ group were significantly different (P lt; 0.001), Ⅲ Ⅰ group rats compared with the above two parameters were not significantly different (P gt; 0.05) . 5, matrine injection on rat ileal mucosa NO Level: ileal mucosa of rats in each group NO concentration: Ⅰ group was 0.25 ± 0.03μmol / g; Ⅱ group was 0.55 ± 0.09μmol / g; Ⅲ group was 0.26 ± 0.02μmol / g; Ⅳ group was 0.28 ± 0.06μmol / g. Group Ⅱ ileal mucosa NO levels were significantly higher than Ⅰ, Ⅲ, Ⅳ group (P lt; 0.001), Ⅳ group NO levels higher than group Ⅰ (P lt; 0.05), Ⅲ group was close to the level of group Ⅰ NO NO levels, no significant difference (P gt; 0.05), Ⅲ and Ⅳ were compared between group differences (P lt; 0.05). Conclusions: 1. Matrine injections can relieve acute radiation enteritis gastrointestinal reactions and pathological changes, suggesting that matrine injection on acute radiation enteritis have a better therapeutic effect. 2 matrine injection can reduce the concentration of NO in the small intestinal mucosa, matrine injection treatment of acute radiation enteritis mechanism of action may be relevant. 3 matrine injection of high doses better than the low-dose group, no adverse reactions.