Screening of Bioactive Compounds in Traditional Chinese Medicine by Open Tubular Capillary Electrochromatography Based on Special Interaction
|Keywords||Blood platelet Specific interaction Filter Traditional Chinese medicine Binding constants|
Chinese medicine is the accumulation of thousands of years of Chinese traditional culture, but also a treasure of the Chinese nation. Chinese medicine because of its complex composition, active components fuzzy limit the pace of its international and modern. How to target drug screening, to identify clear efficacy of the active ingredient has become the key to the development of the modernization of traditional Chinese medicine. Traditional drug screening methods based on the animal model, although this method can be obtained more reliable target active substance, but its period is very long, the cost is very high, also affected by the environment. Receptor theory states that drug screening provides a new way of thinking, receptor target molecule drug screening also came into being, this approach can be combined with a variety of analytical tools, more targeted, and can greatly shorten the screening period, became the more important in today's drug screening method. In this study, platelet established as the target molecule open tubular capillary electrochromatography, on Salvia and thirty-seven-of anti / thrombopoietin active ingredient to filter. To further assess the strength of the interaction force between the platelets and the active ingredient, the present study was first deduced applicable to a method for establishing binding constants calculated, enabling quantitative evaluation of the active ingredient with the interaction of platelets in the complex system. The content of this study are as follows: ① create a new open tubular capillary electrochromatography using physical adsorption platelet fixed to the inner wall of the capillary tube, by changing the fixed length of the migration behavior of the sample in which changes in the mobility and migration degree of platelet Finish length between the linear equation, the binding constant of the target component with platelet interaction. Obtained through the investigation and optimization of the platelet concentration of the eluent, running buffer and other factors, the conditions of application of the present method. Finally, the established method is applied to the evaluation of HSA pazufloxacin interaction, the resulting binding constant is more consistent with literature values, which proves the method can be used for drug - receptor interactions strength evaluation. (2) this method is applied to the positive drug aspirin, negative drug pazufloxacin platelet interactions by fluorescence spectrometry and affinity extraction method validation, proved this method to establish the binding constant formula to specific interactions were evaluated. ③ of this method applied to the nucleoside mixture of anti / screening promote platelet component, the first by the peak change in different platelet coating length of each nucleoside, qualitative screening anti component / thrombopoietin, and then these components separately and platelet experiments obtained with platelet binding constant, so as to quantitatively evaluate the interaction force between these components and platelets. ④ apply this method to the actual Salvia miltiorrhiza and Panax notoginseng extract the anti / thrombopoietin active ingredient Filter, and calculate the binding constant between the peaks corresponding to the component with platelets, by comparing the combination of the size of the constant values, the intensity of these peaks correspond to the components interact with platelets, the reference data for further screening. ⑤ nucleoside composition in nine kinds of wild mushroom / mushroom qualitative and quantitative analysis, the type and content of all wild mushroom / mushroom nucleoside the wild bacteria nucleoside component analysis, preliminary forecast of the potential anti- wild mushroom / mushroom / promote platelet function, laid the foundation for the further exploration of its pharmacological effects.