Study on the Effects of the Mothod of Invigorating the Spleen and Kidney Detoxification and Vigoration of Blood Circulation on Early Diabetic Nephropathy and Flt-1 Expression on Rat Models
|School||Shandong University of Traditional Chinese Medicine|
|Course||Chinese medical science|
|Keywords||Spleen and kidney, the detoxification Huoxuetongluo Law Diabetic nephropathy Comprehensive intervention Ultrastructure Flt-1|
Objective: This paper aims to previous studies , explore spleen and kidney, detoxification the Therapeutic Huoxuetongluo , sugar Shen Ning square with comprehensive clinical intervention role of Chinese medicine , ear early diabetic nephropathy , and is to explore Tangshen Ning treatment of diabetic nephropathy mechanism . Method: 1 . Clinical research : determine the early diagnosis of diabetic nephropathy and Response Evaluation Criteria , main Tangshen Ning Fang , with the method of Chinese medicine , ear and other comprehensive intervention on early diabetic nephropathy , and set the comparison group , using a large sample , prospective, randomized controlled study methods , quality of Life Scale early signs and symptoms of patients with diabetic nephropathy , urinary albumin as the primary efficacy endpoint , clinical observation of safety indicators , and results analysis . Experimental study : triple modeling method ( unilateral renal removal of a small dose of high sugar and fat STZ injection ) the establishment of early diabetic nephropathy rat model , after 12 weeks of drug intervention , biochemistry, electron microscopy , immunohistochemistry of sugar Shen Ning rat model of vital signs , renal function , renal pathology and ultrastructure and Flt-1 immunohistochemical expression . Results: 1 . Clinical research : the comprehensive treatment of Jianpibushenjiedu Huoxuetongluo law not only effective in improving the signs and symptoms of the patients with diabetic nephropathy , improve early renal function ( P lt; 0.05 ) , lower blood glucose and lipid level ( P lt ; 0.05) , but also improve the quality of life of patients , the total effective rate of 90.6% , the indicators are better than the comparison group . Experimental study : triple modeling method can successfully copied the model of early diabetic nephropathy . Tang Shen Ning can significantly improve the survival of the state of the model in rats , early renal function , blood glucose , blood lipids and hemorheology , at the same time also has significant effect on renal pathology and ultrastructural changes , but also to reduce kidney tissue Flt-1 immunohistochemical expression . The conclusion : Jianpibushenjiedu Huoxuetongluo method of early diabetic nephropathy comprehensive intervention effect ; spleen and kidney, detoxification Huoxuetongluo Therapeutic composition Tangshen Ning Fang efficacy exact . Sugar kidney would rather by improving blood sugar , blood lipids and hemorheology protect renal function and kidney pathological changes of the structure has a protective effect , it is worth further study .