Preparation of Conglycinin Enzymatic Peptide and Its Effect on Intestinal Function Immunity in Mice
|School||Northwest University for Nationalities|
|Course||Clinical Veterinary Medicine|
|Keywords||Conglycinin Enzymatic peptide Antibacterial activity Intestinal sIgA Cytokines|
Soybean protein glycinin and conglycinin, the study found that one of the main components of conglycinin contains richer functionality structural areas. This study by several commonly used enzyme purified from soy protein isolate conglycinin digested elect its highest degree of hydrolysis enzyme; inhibitory effect on harmful bacteria in vitro antibacterial tests identified conglycinin ball protease solution peptide; the enzymatic peptides animals through animal trials suppression of harmful E. coli infection, as well as the impact of the immune function of the gastrointestinal tract of animals. Enzymatic studies conglycinin several different sources, the different nature of the enzymatic hydrolysis conglycinin screening the optimum hydrolysis conglycinin protease as an indicator of the degree of hydrolysis. The results show that: the composite the best tool flavourzyme enzyme. Orthogonal experiment determine the flavourzyme enzymatic the conglycinin best hydrolysis conditions: pH value of 7, the hydrolysis temperature of 50 ° C, 14% of the mass concentration of the substrate, concentration of enzyme and substrate ratio by univariate analysis 6%, hydrolysis time of 8h. 2 with conglycinin peptides antibacterial activity flavourzyme conglycinin in different time under the conditions of hydrolysis, the antibacterial activity of the enzymatic solution was measured at different times of the harmful E. coli and Staphylococcus aureus. The results showed that: for 2 h hydrolysis of enzymatic peptide has an antibacterial activity on Escherichia coli and Staphylococcus aureus, the minimum bactericidal concentration of 2.6mg / ml and 3.2mg/ml respectively. Isolated and purified the 2h hydrolyzate by the Sephadex-G15 plate count method to identify the components, and the results prove that the second component antimicrobial activity. 3 of the gastrointestinal immune function in mice with conglycinin peptides selection of clean grade of 50 21-day-old Kunming mice, pre-feeding a week after the official beginning of the experiment. In accordance with the the paired test method is divided into five treatment groups, the healthy control group (I group), infection control group (II group), injection of penicillin group (III group), gavage the conglycinin flavourzyme solution solution group ( Group IV) and orally to conglycinin flavourzyme Hydrolysate (CP2 () V group), the second component of continuous orally administered one week from the second week, in addition to the healthy control group, other groups prepared broth mice at a dose of 0.2ml / gavage, mice were observed during the test of morbidity and mortality in the case, 36 hours after injection of anatomy, spleens were removed, the entire small intestine and feces do determination. Results: The experimental results show conglycinin flavourzyme enzymatic peptide can improve the immune function of the animal body, to resist the invasion of exogenous E. coli infections, the prevention of the occurrence of gastrointestinal diseases.