Dissertation
Dissertation > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology > Chinese medicine Experimental Pharmacology

Effect of Shenfu Injection (SF, Mainly Made up of Panaxoside and Aconite Alkaloid) Which Prevents Kidney from Ischemia/reperfusion Injury and Its Mechanism

Author HuangXiaoGuang
Tutor LiRongShan;LiXueWang
School Shanxi Medical
Course Medical kidney disease learn
Keywords Shenfu injection renal ischemia-reperfusion glomerular filtration rate (GFR) inulin clearance rate renal tubular epithelia calcium overload flow cytometry
CLC R285.5
Type Master's thesis
Year 2008
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Objective Acute renal failure(ARF) secondary to ischemia/reperfusion injury(IRI) remains a significant perioperative problem.The prognosis of ARF is poor(with mortality rates of approximately 50%).This study was designed to investigate the preventive protecting effect of Shenfu injection(SF,its main components are panaxoside and aconite alkaloid) on the renal ischemia-reperfusion injury,and explore its possible mechanism.Methods 120 male Wistar rats(bw:250~300g) were divided into 12 groups randomly(n=10):sham-operated(sham) group,single ischemic(Ⅰ) group,ischemia/reperfusion (IR) 1,24,48,and 72hours groups,each group were divided into the SF-treated group(SHAMs,Is,IR1s,IR24s,IR48s,IR72s)and the control group(SHAMc,Ic,IRlc,IR24c,IR48c, IR72c) further.In SF-treated groups SF injection was injected into peritoneal cavity and in control groups normal saline was used(10ml/kg) once a day for 7 days before the surgery.Then close the double renal arteries of rats in both I and IR groups with forceps for 1 hour,and then reperfused for 0,1,24,48,72 hours respectively,The changes of the serum creatinine(Scr) and the pathological changes of the kidney was studied,the pathological changes of the kidney.Inulin clearance rate was measured by a colofimetric method.Intracellular free calcium([Ca2+]i) signalling in renal tubular epithelial cells were studied using Ca2+ indicator Fluo-3/AM on a flow cytometry.Results Compared with the case in the control groups:①The necrosis of tubular epithelia in SF-treated group significantly decreaed(26.40±13.69%vs 63.83%±16.61%,p<0.01 )When I/R 24 hours;②The rising amount of serum creatinine(Scr) in SF-treated group was diminished after I/R 24h(1.08±0.33 vs 1.36±0.56 mg/dl,p<0.01) and I/R48h(0.72±0.098 vs 1.02±0.36 mg/dl,p<0.05);③Inulin clearance rate of the SF-treated groups was apparently higher than the control groups at I/R1h(0.095±0.026 vs 0.040±0.014 ml/min,p<0.05)and I/R24h(0.135±0.027 vs 0.064±0.041 ml/min,p<0.01);④[Ca2+]i of the renal tubular epithelial cells in the SF-treated group was alleviated(156.57±27.83 vs.213.56±31.24 nmol/ml,p<0.05) When it came to I/R48 hours.⑤The proliferation of tubular epithelial cells in SF-treated group was more active than that in the control group.Conclusion SF injection protects renal structure as well as function against acute renal ischemia-reperfusion injury and accelerates the tubular cell proliferation after renal IR.This experiment validates that SF injection has some preventive protection for the renal ischemia-reperfusion injury and one of its functioning mechanisms could be related to the alleviating the overload of[Ca2+]i.

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