Rat hepatoma evolution of three biochemical factors and related drugs Intervention
|Keywords||Hepatic carcinoma SD rats E-cd β-catenin MMP-7 Kurarinone Selenium yeast|
Objective: To investigate the SD rat liver tissue biochemical factor E-cd, β-catenin and MMP-7 in HCC change each period and oxymatrine combined selenium yeast content changes of the intervention, analyzing the interaction between them and the big Rat liver cancer in rats. Methods: DENA induced cancer agent for SD rat hepatoma become established animal models of different periods. Since the date of carcinogenesis, respectively, in liver tissue 2,4,6,8,10,12,14,16,18 W whichever is appropriate, the use of immunohistochemistry, Wertern blot technique in liver tissue of rats was measured above three biochemical factor content change. Results: ① pathological findings: induced cancer rats 2-4W showed mild inflammatory response, 6W showed severe inflammatory response ,8-10W as adenomatous hyperplasia ,12-14W with atypical adenomatous hyperplasia, 16-18W bile duct carcinoma. ② Immunohistochemistry: carcinogenesis group E-cd-positive rate with prolonged carcinogenesis gradually decreased to adenomatous hyperplasia period was significantly lower than the control group (P lt; 0.05); intervention group E-cd's decreasing trend slower than induced cancer group, the positive area of ??the region and increased color depth varying degrees, but no significant difference (P gt; 0.05). Carcinogenesis group β-catenin-positive rate with decreased cancer inducing prolonged from inflammatory reaction stage to tumor formation were significantly lower than the control group (P lt; 0.01); intervention group β-catenin levels in the medium term and induced carcinogenesis The positive rates of cancer, there are some differences, but later reduced the difference, no significant (P gt; 0.05). Induced cancer group the positive rate of MMP-7 with the process of carcinogenesis gradually increased to the stage of atypical adenomatous hyperplasia was significantly higher (P lt; 0.01), to tumor formation, the differences were highly significant (P lt; 0.01 ); intervention group positive rate of MMP-7 in the inflammatory response induced cancer group stage was essentially flat; from atypical adenomatous hyperplasia stage to biliary epithelial carcinoma MMP-7 positive rate gradually increased, but induced cancer group had no significant difference (P gt; 0.05). ③ Wertern blot: carcinogenesis group E-cd content LIVER gradually reduced during the bile duct carcinoma was significantly lower than the control group (P lt; 0.05); intervention group E-cd content at all times slightly induced cancer group and in atypical adenomatous hyperplasia was significantly higher than that induced cancer group (P lt; 0.05). Carcinogenesis group β-catenin levels from severe inflammatory response times to generate a very significant difference cancer than the control group (P lt; 0.01); intervention group mid-β-catenin levels were lower than that induced cancer, severe inflammatory response in the period to generate cancer showed a significant difference (P lt; 0.05), atypical adenomatous hyperplasia was significantly different periods (P lt; 0.01). Conclusion: The rat hepatoma evolution E-cd, β-catenin decreased gradually, MMP-7-positive rate was gradually increased, and the presence of ectopic expression of β-catenin. Oxymatrine combined evolution of selenium yeast intervention liver after liver tissue cytologic features and E-cd, β-catenin and MMP-7 expression were some changes. E-cd, β-catenin and MMP-7 protein factor in these three kinds of liver cancer evolution and drug intervention there are some changes under the law, which for the study of the pathogenesis of liver cancer, liver cancer finding new markers and therapeutic targets provided a theoretical basis.