Dissertation > Medicine, health > Surgery > Urology ( urinary and reproductive system diseases) > Men 's sexual dysfunction > Male infertility

The Expression of CaV1.3 and Ryrs1 in the Corpus Cavernosum Smooth Muscle of Aged Rats and Related Research

Author LuoYi
Tutor JiangZuo
School Luzhou Medical College
Course Department of Urology
Keywords Erectile dysfunction (ED) Calcium channel (CaV1.3) Ryanodine receptor (Ryrs1) Old Corpus cavernosum
CLC R698.2
Type Master's thesis
Year 2009
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Objective: To have a higher prevalence of erectile dysfunction (erectile dysfunction, ED) have a great impact on the quality of life of its men and their partners. With the aging population, the proportion of the total population of the community growing number of aging patients with ED has also increased. , Massachusetts Male Aging Study (MMAS) reports that, in a random sample of 40-70 year-old respondents in ED prevalence as high as 52%. Recent studies have found that certain diseases is the result of genetic defects and function of ion channels variation, called ion channel disease. Although many diseases does not belong to the ion channel disease have a close relationship, but a link or stage of disease and dysfunction of ion channels, and also found that many drug prevention and treatment of disease by acting on ion channels onset. Therefore, the physiology and disease of ion channels is becoming one of the hot spots of the current research. Corpus cavernosum smooth muscle relaxation or contraction regulation cavernosum smooth muscle tension physiology decided penile erection or weak state. Many evidence suggests that other vascular tissue, potassium channels, calcium channel corpus cavernosum smooth muscle tension major regulator. Cavernosum smooth muscle cells cultured free cavernous tissue muscle strips research: Most of the neurotransmitters involved in penile erection by the smooth muscle cells of the role of ion channels and ion transmembrane flow regulation cavernosum smooth muscle tension. The present study, the Ca2 channel. Studies have shown that L-type Ca2 channel Cav1.3 Ca2 transporter play an important role in Ca2 channel receptor ryanodine receptor (RyR) three subtypes RyR1, RyR2, RyR3 in the regulation of Ca2 also played important role in this subject through animal studies of L-type calcium channels (CaV1.3) and ryanodine receptor RyR1 in the penis of aged rats rat corpus cavernosum expression to explore the the aging erectile function obstacles (ED) mechanism to provide the basis. Methods: A group (2 months old) and group B (18 months old) male SD rats were each 10 measured serum testosterone, using RT-PCR and immunohistochemical methods the two groups CaV1.3 and RyrR1, penis sponge Expression of body. Results: A group average serum testosterone (T) to 274.426 ± 96.371ng/dl 135.642 ± 42.431ng/dl group B, B serum testosterone decreased significantly compared with group A (P <0.05). Immunohistochemical data using the integrated optical density (IOD) for semi-quantitative analysis: B group CaV1.3 expression (IOD = 18.65 ± 8.47) compared to the A group (IOD = 75.48 ± 14.28) significantly reduced (P <0.05); of RyR1 expression group (IOD = 21.37 ± 9.64) compared with group A (IOD = 78.23 ± 13.57) was significantly reduced (P <0.05). The CaV1.3 and RyR1mRNA average gray value expressed by the two groups of rat corpus cavernosum comparison: B the group CaV1.3mRNA expression (mean gray value = 0.382 ± 0.046) compared to group A (mean gray value = 1.137 ± 0.415) was significantly lower (P <0.05); the B group RyR1mRNA expression (average gray value = 0.146 ± 0.053) compared to group A (mean gray value = 1.215 ± 0.261) was significantly lower (P <0.05). The conclusion: CaV1.3 and reduced expression in the aged rat corpus cavernosum Ryrs1 may be one of the mechanisms of aging of ED occurred.

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