Dissertation
Dissertation > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology > Chinese medicine Experimental Pharmacology

The Protective Effect of Garlic Polysaccharide on Chronic Alcoholism Liver Injury in Mice

Author LiangXiangZhen
Tutor ChenJing
School Jinan University
Course Physiology
Keywords Garlic Polysaccharide Chronic alcoholism Liver injury Antioxidant
CLC R285.5
Type Master's thesis
Year 2010
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The research background alcoholism, chronic alcoholism has been the world's most serious social and public health problem. Alcoholic liver disease (Alcoholic Liver Disease, ALD) is due to the long-term liver damage caused by heavy drinking and its series of lesions, chronic alcoholism is the most common and one of the most serious complications. Also the 10 most common causes of death among Western countries and lead to cirrhosis of the most important reason. In recent years, as China's rapid economic development and the improvement of people's living standards, the incidence of liver damage caused by alcohol showed an increasing trend, has become the second largest after the viral hepatitis liver disease, a serious threat to the national health, is in urgent need of medical study and resolve issues. Clinical studies have confirmed that alcoholic liver disease is a progressive development of the irreversibility of the pathological changes, clinical no cure. The study effective methods of prevention and early treatment of liver injury, has important clinical value and social significance of reducing groups in the incidence of alcoholic liver disease, and guide appropriate drug development. On alcoholic liver disease pathogenesis studies suggest that the toxic effects of alcohol and its metabolites in the liver, and metabolic processes of oxygen radical formation, the antioxidants GSH depletion, lipid oxidation and activation of multiple cytokines produce alcoholic The main mechanism of liver injury and fibrosis, and proposed to oxidative stress and lipid peroxidation in the center of the \But radical pathogenesis of alcoholic liver disease, the applied natural plant antioxidants intervention alcohol liver injury is rarely reported. Garlic Polysaccharide effective active ingredient extracted from garlic, belonging to the daisy-carbohydrate fructan, domestic research confirmed that the garlic polysaccharide with antioxidant, anti-viral, mediation lipids, lower blood sugar, enhance immunity, and treatment of viral myocarditis, improve CCL4 caused liver damage and other effects. Prevention and treatment of liver disease drug development prospect is very or health food, but about garlic polysaccharide of animal and human chronic alcoholic liver injury intervention studies have not been reported. The purpose with chronic alcoholism, liver injury model in mice, observed protective effect of garlic polysaccharide on mice with chronic alcoholism, liver damage, and explore its mechanism from the point of view of antioxidant research, prevention and early treatment of garlic polysaccharide developed into chronic alcoholic liver disease, drug or health food to provide the basis of scientific experiments. KM mice (70) by weight and sex were randomly divided into five groups of 14 each, are as follows: normal control group (NC), model group (MC) and garlic polysaccharide high dose group (GH 200mg/kg / d), the middle dose group (GM 150mg/kg/d,), the low-dose group (GL 100mg/kg/d). Normal control group with distilled water, the rest of the group in order to gradually increasing doses, with the 56 ° Hongxing of Erguotou liquor 0.05mL/10g.d gavage 3 days instead 0.08mL/10g.d dose of alcohol toxicity five days after the change alcohol toxicity 0.10mL/10g.d dose one week last increased 0.12mL/10g.d dose of alcohol toxicity to the day before the end of the experiment, with the impact of 0.14mL/10g.d dose liquor gavage. Day before alcohol toxicity 30min, the control group and the model group with distilled water, the other experimental groups with different doses of garlic polysaccharide gavage eight weeks of continuous experiment. 8 weeks mice weighed weight, pull the eye to take blood, centrifuged to obtain serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the activity assay, dislocated mice were killed at the same time, the liver weighed, and immediately take part of the liver tissue, fixed with 10% formalin solution histopathological examination of the liver to be done. The rest of the liver tissue to ice saline wash was homogenized liver tissue malonyl aldehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD), glutathione peroxide matter enzymes (GSH-Px), sodium - potassium-ATP enzyme (Na-K-ATP) and alcohol dehydrogenase (ADH) activity was measured. 1, chronic alcoholism general condition of mice model mice from three weeks of the experiment began drinking increased salivation symptoms, scratching action. Four weeks, the body stiff drinking 20 minutes after the show, sluggish, listlessness and other symptoms, usually after 2-3 hours recoverability. 6 to 7 weeks, the mice after drinking a staggering gait, drowsiness and other obvious symptoms of chronic alcoholism, recovery time, an average of 4 to 6 hours. Comparison with normal mice, the model mice, poor appetite, slow weight gain, fur gloss differential. Compared with the model group, the garlic polysaccharide each intervention group mice drunkenness symptoms are relatively mild hangover relatively short period of time, an average of 2-3 hours, of appetite and fur gloss relatively better model group. The results showed that garlic polysaccharide has a role to improve the symptoms of chronic alcoholism in mice. 2, changes in body weight and liver weight index of each experimental group of mice compare the body weight of mice in each experimental group 1 to 3 Zhou Chengming significant gradual upward trend, Week 4 model mice weight began to fall, the first five weeks weight rebound slightly increased weight after six weeks significantly decreased steadily, final body weight was significantly lower than the normal group (P lt; 0.05), indicating that the long-term excessive drinking will affect mice appetite, resulting in malnutrition. Compared with the model group, the garlic polysaccharide, the high dose group the body weight of mice after five weeks began to steadily decline is not obvious, final body weight was significantly higher than that in the model group (P lt; 0.05) the garlic polysaccharide low dose group weight fluctuations However, compared with the model group, the weight loss is not obvious, the results suggest that garlic polysaccharide to improve the role of mice appetite. Liver weight index compared with the normal group, the model group was significantly higher (P lt; 0.05), significantly lower than the model group (P lt; 0.05) and garlic polysaccharide in each experimental group mice liver weight index results suggest that garlic polysaccharide on alcoholic liver injury protection role. 3, each experimental group, serum ALT, AST activity and liver activity comparison with the normal group, model group, serum alanine and aspartate aminotransferase activity was significantly higher (P lt; 0.05), the liver ATP enzyme activity was significantly reduced ( P lt; 0.05), ADH1 and ADH2 activity significantly increased (P lt; 0.05), indicating that long-term excessive drinking can cause serious damage to the mouse liver; prompted continuous intake of ethanol induced liver ADH activity increased acetaldehyde increased aggravate the liver damage; confirmed liver injury in mice model of chronic alcoholism success. Compared with the model group, the garlic polysaccharide dose mice serum alanine and aspartate aminotransferase activity was significantly lower (P lt; 0.05), ATP enzyme activity was significantly higher (P lt; 0.05); compared with the model group, garlic polysaccharide each ADH1 activity of the dose groups were significantly reduced, most obvious in both dose groups; garlic polysaccharide lower middle dose group ADH2 activity, to the high-dose group was significantly, but the low-dose group intervention had no significant effect. Has a protective effect, the mechanism may be related to increased chronic alcoholism mouse liver ATP activity, inhibit ADH activity increased effects of chronic alcoholism, liver damage in mice confirmed the garlic polysaccharide. 4, the livers of mice in each experimental group oxidation and antioxidant levels compare with the normal group compared with model group mice was significantly higher liver MDA content, GSH content was significantly reduced (P lt; 0.05), SOD and GSH-Px activity of antioxidant enzymes significantly lower (P lt; 0.05), indicating that chronic alcoholism mouse liver injury and its in vivo oxidation and antioxidant imbalance related. Compared with the model group, the garlic polysaccharide, MDA content of the high-dose group was significantly lower (P lt; 0.05), the best effect of middle dose group, but the low-dose group reduce not statistically significant (P gt; 0.05); each dose group GSH content increased significantly (P lt; 0.05), SOD and GSH-Px activity of antioxidant enzymes improved significantly (P gt; 0.05), and showed a dose-response relationship. Prompted the Garlic Polysaccharide on the protective effects of chronic alcoholism, liver damage in mice and its increase antioxidant capacity. 5, in each experimental group of mice liver histopathological changes compare liver biopsy by light microscopy observations show clear normal mice lobular architecture, multilayer liver cells were mononuclear cells arranged in neat rows, no degeneration and necrosis, sinusoidal no expansion of the congestion, no inflammatory cell infiltration of the portal area; model mice markedly swollen liver cells, part of the region of ballooning; the hepatic lobule blurring, liver cells showed vesicles steatosis, occasional steatosis , portal area and inflammatory cell infiltration, and necrosis within the liver lobule increased significantly, but the liver sinusoidal around no fibrosis-like changes, indicating that for 8 weeks ethanol feeding has caused liver damage in mice, pathological changes in fat and inflammatory lesions stage . Confirmed successful liver injury in mice model of chronic alcoholism. Compared with the model group, the garlic polysaccharide in each intervention group hepatocyte swelling alleviate some hepatocytes mild edema, occasional small focal liver cell steatosis, lobular and periportal area no inflammatory cell infiltration, the lesions improved significantly, to the garlic polysaccharide improvement in the high-dose group, confirmed that the the garlic polysaccharide with reversal of hepatic steatosis, reduce inflammation, and repair of the role of the liver cells. The conclusions of the long-term sustainability of excessive drinking can lead to alcoholism and liver of mice with chronic serious injury; the garlic polysaccharide protective effects of chronic alcoholism, liver damage in mice, the mechanism of their enhanced antioxidant capacity and free radical scavenging and inhibition of hepatic ethanol off The hydrogen activity increased role.

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