Dissertation > Medicine, health > Pharmacy > Pharmacopoeia Codex ( formulary ),drug identification > Drug identification > Determination of drug content

Study on Detection Method of Gatifloxacin and Sulfadiazine

Author ZhangLei
Tutor DongXueZhi
School Henan University
Course Analytical Chemistry
Keywords Spectrographic Method Detection Gatifloxacin Surfactantsa
CLC R927.2
Type Master's thesis
Year 2010
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Pharmaceutcial quality directly affects the sufferers’life health and safety. Therefore, Pharmaceutcial quality must be controlled comprehensively in order to ensure its safty, reasonableness, validity when it is used.Nowadays, the trend of pharmaceutcial analysis is to determine the trace pharmaceutcial ingredient of the complex samples simply and reliably. Therefore, Spectrographic method has become one of the most essential and important methods in the determination of pharmic products due to its easy apparatus, simple procedure, high selectivity and sensitivity.The aim of this dissertation is to explore simpler, more sensitive determination methods of Gatifloxacin(GFLX) and Surfactantsa(SD), with using spectrophotometry as research means. The major contents are described as follows :1.A somewhat through and all-around review of the application of methods in the analysis of Gatifloxacin and Surfactantsa is given in this part.2.Established a fluorometry for the determination of GFLX:The interaction between Congo Red(CR)and Calcein in pH 6.00Britton-Robinson buffer solution. resulted in a fluorescence intensity of Calcein decreasement. Addition of GFLX into the Calcein-CR system, the fluorescence intensity was enhanced. This method has been applied for the determination of GFLX in tablet, injection and urine, and the results were very close to those obtained by the UV method reported.3.Simultaneous determination of Gatifloxacin and Levofloxacin by spectrophotometry and measurement analysis of two lines with same one point.The Al3+ and Sodium Dodecyl Sulfate (SDS) could react with Gatifloxacin and Levofloxacin to form ion-association complexes, their UV absorption spectra were located at 292 nm and the difference was their linear amplification different as their Concentration. It is based on the difference a novel method for simultaneous determination of Gatifloxacin and Levofloxacin had been developed with“measurement analysis of two lines with one same point”.4.Researched the alizarin red-Al3+-SD system in different acidity.SD can enhance a UV absorbance of ARS, addition of Al3+ into the ARS-GFLX system, the absorbance was much stronger. Then maximum absorbance at 261nm in pH 4.50 Britton-Robinson buffer solution and at 259nm in pH 10.00. So the contents were calculated according to the standard curves. In practical work,we can determine SD use appropriate method in pH 4.50 or in pH 10.00 by required. Both of methods have been applied for the determination of SD in tablet and urine, and the results were very close to those obtained by the fluorimetry method reported.5.Investigated SD by its reaction with XO develop the color system in the presence of surface active agent CPC or CTAB. Study most of surface active agents, such as positive ions, negative ions and non ions. The study shows that CPC and CTAB have the most enhance function. So established two methods of determine SD.XO -SD- CPC and XO -SD- CTAB color systems have the special feature of good inrapidily seasieivity and stability. Used in the survey of the content of SD in the tablet.The results are satisfied.

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