Expression of MMP-9 and TIMP-1 in Hyperhomocysteinemic Rats
|Keywords||High homocysteine Atherosclerosis MMP-9 TIMP-1 Folate Vitamin B12|
Objective: To determine the high homocysteine ??(HHcy) serum matrix metalloproteinase -9 (MMP-9) and tissue inhibitor of matrix metalloproteinase -1 (TIMP-1) expression was observed folic acid, Vitamin B12 (VitB12) fed on high methionine caused HHcy and atherosclerosis (As), and to explore the pathogenesis of HHcy caused by As. Methods: 30 male Wistar rats were randomly divided into control group, methionine group and the intervention group. Control group was given normal diet; methionine group of ordinary diet supplemented with 2% methionine feeding, while more than two groups were given daily 2ml normal saline; intervention group ordinary diet supplemented with 2% methionine feeding, both given daily folic acid 0.5mg, VitB12 25ug dissolved in 2ml water gavage, experimental period was 8 weeks. By ELISA serum homocysteine ??(Hcy), MMP-9 and TIMP-1. With HE staining of rat carotid artery pathology. Results: (1) with the control group, serum Hcy (11.63 ± 4.36umol / L) compared to the intervention group Hcy (16.21 ± 4.41 umol / L) and methionine group Hcy (20.57 ± 4.34umol / L) serum concentrations were significantly higher, P lt; 0.05, compared with the intervention group, methionine significantly increased serum Hcy, P lt; 0.05. (2) with the control group, serum MMP-9 (4.63 ± 0.82ng/ml) compared to the intervention group MMP-9 (5.72 ± 0.68 ng / ml), methionine group MMP-9 (6.59 ± 1.04 ng / m) serum concentrations were elevated, P lt; 0.05, compared with the intervention group, methionine group was significantly higher serum MMP-9, P lt; 0.05. (3) with the control group, serum TIMP-1 (1179.53 ± 243.76pg/ml) compared to the intervention group TIMP-1 (1438.83 ± 185.63 pg / ml), methionine group, TIMP-1 (1640.73 ± 184.40 pg / ml) serum concentrations were significantly higher, respectively, P lt; 0.05, compared with the intervention group, methionine elevated serum TIMP-1, P lt; 0.05. (4) in the control group (r = 0.825, P lt; 0.01), the intervention group (r = 0.963, P lt; 0.01) and methionine group (r = 0.860, P lt; 0.01) in Hcy and MMP-9 was positively correlated ; in the intervention group (r = 0.950, P lt; 0.01) and methionine group (r = 0.547, P lt; 0.01) in Hcy with TMP-1 was positively correlated, whereas in the control group (r = 0.255, P gt; 0.05) in Hcy and TIMP-1 there is no correlation between. (5) were observed in the methionine group carotid artery smooth muscle cell proliferation disorder, elastic fiber blend. Compared with the control group, the intervention group carotid artery wall no obvious abnormalities. Conclusion: HHcy make MMP-9 and TIMP-1 expression is increased and destroy MMP-9 and TIMP-1 balance between, prompting the extracellular matrix (ECM) degradation and re-distribution, causing vascular wall remodeling, may be As one of the mechanisms induced HHcy. Folic acid and VitB12 can effectively reduce Hcy, the occurrence of As played a preventive role.