The Impact and Significance of Expression of Pinellia Ternate Polysaccharide Combined with Capecitabine on CT-26 Tumor Cell MHC-Ⅱ

Purpose mice transplanted colon adenocarcinoma CT-26 animal models, relatively saline the Pinellia polysaccharide, Xeloda, Pinellia polysaccharide combined with Xeloda inhibition of mouse CT-26 subcutaneous xenografts flow cytometry to detect different groups of tumor-bearing mice peripheral blood CD4, CD8 changes as well as different groups of tumor tissue cell surface MHC-Ⅰ, MHC-Ⅱ expression in order to better provide for the clinical objective and scientific basis. Method 1. Improved water extraction and alcohol precipitation method extract the Pinellia polysaccharide. 2.BALB / C mice 32, CT-26 colon adenocarcinoma cell armpit after planting modeling success, were randomly divided into: normal saline group, the Polysaccharide low dose group, middle dose group of Polysaccharide and Polysaccharide high-dose group, n = 8, were given different interventions: saline group were given saline 0.2ml daily gavage, 6 times / week; polysaccharide low dose group to Pinellia polysaccharide 0.2ml gavage, the dose per day for 100mg/kg, 6 times / week; polysaccharide middle dose group given Polysaccharide 0.2ml orally daily dose of 200mg/kg, 6 times / week; polysaccharide high dose group was given the Polysaccharide 0.2ml orally at a dose of daily of 400mg/kg, 6 / weeks. Collected from each group, tumor size at the end of the two weeks experiment weighed inhibitory rate was calculated, the dose-response relationship observed with different doses of Polysaccharide draw Polysaccharide gavage dose. BALB / C mice 24, CT-26 colon adenocarcinoma cell armpit after planting modeling success, were randomly divided into: the saline group Polysaccharide group, chemotherapy group, Pinellia polysaccharide combination chemotherapy group were given different intervention: saline group were given saline 0.2ml orally daily, 6 times / week; the Pinellia polysaccharide group be Pinellia polysaccharide 200mg/kg / day orally, 6 times / week; chemotherapy group was treated with Xeloda irrigation stomach daily 267mg/kg, 6 times / week; Pinellia Pinellia polysaccharide combination chemotherapy group was given polysaccharide 200mg/kg to to Xeloda 267mg/kg orally. Mice in each group peripheral venous blood collected 2 weeks the end of the experiment, the surgical removal of the tumor inhibitory rate was calculated. 4. Flow cytometry in peripheral blood CD4, CD8 changes and tumor tissue MHC-Ⅰ and MHC-Ⅱ expression. Results of a modified water extraction and alcohol precipitation method can be more thoroughly extracted a relatively pure pinellia polysaccharide the Pinellia polysaccharide yield of 1.92%, the the Pinellia polysaccharide purity of more than 91%. Successfully established animal models of mice transplanted colon adenocarcinoma CT-26. Was found, the inhibition rate of change with the increase of the dose of Polysaccharide was the dose-effect relationship, Polysaccharide 200mg/kg optimal dose. 4. Yiliu higher than the combined treatment group Xeloda monotherapy group, there is a significant difference (P lt; 0.05). Flow cytometry medication group CD4% and significantly higher than the saline group CD4%, there is a significant difference in P lt; 0.05, the drug group was no statistically significant difference P lt; 0.05. CD4 / CD8 of treatment group was significantly higher than the saline group, there is a significant difference between P lt; 0.05; treatment group (CD4 / CD8) the Polysaccharide combined Xeloda Xeloda group than in groups, there is a significant difference between P lt ; 0.05. 6 MHC Ⅰ, medication and saline groups have higher expression; expression of MHC II molecules the Pinellia Polysaccharides Joint Xeloda and pinellia of polysaccharide group, Xeloda group, saline group there are significant differences in (P lt; 0.01). Conclusion 1. Pinellia polysaccharide joint Xeloda animal model of peripheral blood CD4 / CD8 ratio can be increased. 2. Polysaccharide joint Xeloda may promote animal model of MHC-II molecule expression. Above prompted Pinellia the polysaccharide may promote Xeloda inhibitory effect, the mechanism may increase the expression of the mouse adenocarcinoma MHC-II molecules, Polysaccharide enhance the effects of the tumor-bearing mouse cells immune.