Dissertation
Dissertation > Industrial Technology > Light industry,handicrafts > Food Industry > Brewing industry > Brewing Industry > By-product processing and utilization

Study on Extraction of β-1, 3-Glucan from Alcohol Yeast in Mature Fermented Molasses Mash

Author WeiTao
Tutor LiuHuiXia
School Guangxi University
Course Sugar works
Keywords Alcohol Mash Reuse rate Yeast extract β-1, 3 - glucan
CLC TS261.9
Type Master's thesis
Year 2008
Downloads 164
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The 21st century is the era of the resources and environment, rational use of resources, protection of the environment is an inevitable requirement for sustained development. The subject to comprehensive utilization as the guiding ideology, first proposed changing the process from the alcohol production at source, recycling alcohol yeast mash-generation of high value-added products, alcohol and yeast alcohol waste yeast turning waste into treasure, increase economic benefits. Can improve the reuse rate of liquid waste, but also reduce wastewater pollution load, to reduce the cost of sewage treatment and comprehensive utilization explore new effective way for the adjustment of product structure and byproducts of the sugar industry, to provide a practical basis for industrial production. The project studied the basic theory and the aspects of process conditions, briefly summarized below: 1. Determine the the matured mash centrifugal process conditions as well as yeast mud chemical scrubbing process conditions: 30% of the wash water, washed 3 times, revolutions 3000r/min centrifugal centrifugation time 10min; The yeast slurry chemical wash first and then 0.1% sodium bicarbonate 1.75% tartaric acid washing 30min. Under these conditions, the alcohol yeast yield reached 1.5%, the matured mash Alcohol loss of 3%. The total sugar content of the resulting alcohol yeast than yeast. 2. Detection compared the differences of the conventional process and process waste technical indicators, and test the waste liquid instead of water reuse rate. A 53.3% decline in new process alcohol wastewater SS decreased by 34%, the COD, BOD decreased by 56.9%, greatly reducing waste governance is difficult. Alcohol waste instead of the new process water reuse rate of up to 20%, 4 times than the conventional process wastewater reuse rate the yeast washing liquid waste (1) recycling rate of 70%. Alcohol yeast as raw materials, the study identified autolysis - Ultrasonic - enzymolysis - alkali dissolved oxygen method. Optimization of β-1, 3 - glucan preparation process, to achieve β-1, 3 - glucan and yeast pumping Extracts from cogeneration. Single factor and orthogonal experiments to determine the most important factors affecting polysaccharide purity, and then use the response surface methodology derived enzyme dosage A temperature of the alkali-soluble B concentration of alkali-soluble C of three factors polysaccharide purity (Y) between regression equation: Y = -1174.3175 0.1755 × A 27.3212 × B 59.3075 × C-6.75 × 10 -4 × AB-4.5 × 10 -3 × AC -0.253 × BC-1.1267 × 10 -4 × A 2 -0.1606 × B 2 -5.6467 × C 2 determine the optimum conditions: the enzyme dosage 470U / g, 3.2% of the concentration of alkali-soluble alkali melting temperature of 81.5 ° C. Under optimum conditions, the yeast extract was 69.92% polysaccharide rate of 15.02%, β-1, 3 - glucan content of 76.21%, 1.52% protein content. Brewer's yeast, active dry yeast as raw materials and alcohol yeast polysaccharide extraction experiments by comparing the product components learned: In this study, the extraction process is also applicable to the brewer's yeast and active dry yeast; alcohol yeast β-1 3 - the total glucan higher than beer yeast and active dry yeast, proved alcohol yeast extract β-1, 3 - glucan has great development potential. Of zymosan samples physicochemical properties, initial purity identification, monosaccharide composition analysis and molecular structure analysis, the results show that: the the zymosan sample is insoluble in water and many organic solvents, in dimethyl sulfoxide to form a stable suspension, the products offered by the single component of glucose, β-glucan branching degree of about 21%, and that prepared in this experiment β-1, 3 - glucan has high biological activity.

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