Egg Formation Process Ultrastructure and Calcium Deposition Related Gene Expression Studies
|Keywords||Eggshell Quantitative PCR Calcium deposition Ultrastructure Gene|
Poultry eggshell to protect the contents of the egg is not compromised, and a very important factor to reduce broken egg preservation, packaging and transportation. Study laying hens, to slaughter hens whichever the eggshell their uterus at different time periods of the egg into the uterus, using X-ray spectrometer, a scanning electron microscope to explore the elements of the eggshell observed inside and outside surface ultrastructure of the egg into the uterus before and after different stages of uterine, the total RNA using Trizol method, and reverse-transcribed into cDNA, using fluorescence quantitative RT-PCR gene eggshell calcification calcium binding protein d28K (Calbindin -D28k), osteopontin protein (OPN), the calcium ions ATP enzyme (CAATP), carbonic anhydrase enzymes (CA), ovalbumin (OV) in the uterine expression pattern of the different time periods studied, combined with a variety of gene expression abundance analysis of eggshell calcification is closely related gene, calcium deposition relationship to find the basis for further study of its eggshell calcification. Draw the following results: 1. This article from the experimental analysis of X-ray energy dispersive spectroscopy and scanning electron microscopy to explore the eggshell elements, the ultrastructure of the eggshell formation of calcium deposits in the relationship. Draw more and more calcium deposition in the process of egg formation, the 5h period calcium deposition hour before the egg into the uterus to the egg into the uterus up after less and less the amount of calcium deposition. By real-time PCR analysis of the expression pattern of the OPN.OV different time periods in the uterus, in the hours before the egg into the uterus, the egg into the uterus 1h, egg into the uterus after 5h OPN, OV expression levels detected gradually increased, the egg into the uterus after 15h, 1h egg after egg after 2h gradually decline. Analyzed the expression pattern of CAATP different time periods in the womb detected in the hours before the egg into the uterus, hour before egg into the uterus, the egg into the uterus, 5h, the egg into the uterus after 15h, egg production after 1h CAATP The expression levels gradually increased in the egg after 2h decreased. Calbindin-D28k different time periods in the womb expression pattern drawn from the analysis, in the hours before the egg into the uterus, hour before egg into the uterus, the egg into the uterus, 5h, 15h Calbindin-D28 expression levels in the egg into the uterus gradually rises high, drawn from the analysis of the expression pattern of the different time periods of the CA in the womb, and in the hours before the egg into the uterus, the egg into the uterus 1h, egg into the uterus after the egg after 1h, 2h egg after another declined 5h CA expression gradually increased after the egg into the uterus after 15h, 1h egg after egg after 2h gradually decline. 4 of this study indicate that OPN OV key genes affect the formation of the egg white, while CAATP.Calbindin-D28, CA key genes that affect the deposition of calcium.