Dissertation > Medicine, health > Clinical > Therapy > Physical therapy,naturopathy

Inhibitory Effect of Electrical Stimulation on GABA-induced Depolarization in Rat Dorsal Root Ganglion Neurons

Author ZhangJing
Tutor SiJunQiang
School Shihezi University
Course Physiology
Keywords Electrical stimulation DRG GABA Depolarization Pain
CLC R454
Type Master's thesis
Year 2008
Downloads 57
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Objective: In this study, dorsal root ganglion (Dorsal root ganglion, DRG) as a model, the application of different frequency electrical stimulation in the rat sciatic nerve distal to observe the electrical stimulation of GABA-mediated DRG neurons membrane potential depolarization role of electrical stimulation on the DRG nociceptive information transmission. : Freshly isolated rat DRG sciatic nerve specimens application intracellular microelectrode recording technique, given by the the Powerlab bio-signal acquisition and processing analysis system (stimulation pulse width from 0.02 to 5 ms stimulus intensity V) voltage stimulation, stimulus intensity higher than the threshold intensity. Guide the action potential, the determination of the action potential conduction velocity (Conduction velocity, CV) Judging from the size of the cells and nerve fibers excited categories. On this basis, to the pulse width 5 ms, the strength of 2 V, the frequency for 1 Hz, 2 Hz, 5 Hz, 10 Hz and 50 Hz pulse wave distal sciatic nerve in rats, observed stimulation before and after the GABA (3 x 10 -4 mol / L) DRG neurons mediated membrane potential to changes in the polarization response amplitude. Results: 1. Nerve fiber conduction velocity measurement: experimental 40 CPC cell to guide the action potential, of which 70% (28/40) were Aδ fiber, 30% (12/40) of the Aβ fiber. 2 each frequency stimulation in different time response amplitude before stimulation Compare: 5 frequency groups are 2 min after the stimulation can lead to the inhibition of GABA-mediated DRG neuron membrane to the polarization response. Different frequency groups inhibition type, the amplitude of the maximal inhibition time is also different. Frequency of 1 Hz, 2 Hz, 5 Hz, 10 Hz and 50 Hz, respectively, stimulation after 2 min, 2 min, 24 min, 12 min and 2 min maximal inhibition, inhibition rates were 16.78% ± 3.64%, 19.80% ± 3.80 %, 24.68% ± 3.99%, 29.20% ± 3.36% and 20.44% ± 3.12% (n = 4 to 5); its inhibitory effect duration to stimulation after 24 min, 24 min, 48 min,> 72 min and 48 min, 10 Hz inhibition of the duration of the longest. 3. The same time period different stimulation frequencies group comparison: the same time period after the stimulation frequency group of GABA-mediated DRG neurons membrane potential depolarization inhibition of the reaction amplitude comparison, found to stimulate end of 2 min, 12 min, 24 min After 36 min, 48 min, 60 min and 72 min after 5 Hz group and the group of 10 Hz electrical stimulation of GABA-mediated DRG neuron membrane depolarization stronger inhibitory effect in several other set of frequencies between the two groups Compare 10 Hz groups strong inhibitory effect. Conclusion: The frequency electrical stimulation of the sciatic nerve, can inhibit DRG neurons membrane GABA depolarization and 10 Hz electrical stimulation of the strongest inhibition. The mechanism may be electrical stimulation to activate the nociceptive Aδ fiber nociceptive information to upload. Electrical stimulation of the GABAA receptor phosphorylation and inhibition of GABA-mediated presynaptic inhibition, to promote excitatory neurotransmitter release, the easy introduction of nociceptive information, leading to pain.

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