Research Yiqihuoxuejiedu law ulcerative colitis rat gastrointestinal hormones on the
|School||Beijing University of Traditional Chinese Medicine|
|Course||Chinese medical science|
|Keywords||Ulcerative colitis Gastrointestinal peptide hormones Formula of Kuijiefufa Mechanism of action|
ObjectUlcerative colitis (UC) is a chronic non-specific inflammatory disease with rectum and colon that the pathogeny of the disease is not clear yet. Its clinical manifestations are abdominal pain, diarrhea, mucus, pus and blood stool, tenesmus and so on. In recent years, there is an increasing tendency of incidence of this disease in China year by year, and because it is hard to cure, the reoccurrence rate is high, the prognosis is poor, it is regarded as one of the hardest curable disease by the World Health Organization. On the understanding of the pathogenesis of UC is mainly concentrated in the infection, immune, genetic and other aspects. Gastrointestinal peptide hormones widely distributed in the gastrointestinal mucosa and intrinsic nervous system. It plays an important role in the gastrointestinal smooth muscle movements, gland secretion, blood supply, and local inflammatory cells, immune cells and cytokines. In recent years, the researches on the gastrointestinal hormone involved in the pathogenesis of ulcerative colitis are gradually increasing, but the research on its role in the recurrence of UC are few.Professor Wang Xinyue, my tutor has concluded some predecessors’ valuable experience, combined Chinese traditional medical theory and modern progress in medical research, created Kuijiefufafang which based on treating with tonifying Qi, promoting blood flow and detoxifying Du through years of clinical practice. It has been made on the good clinical efficacy and reduce the relapse rate significantly. The UC rat models were intervented by the Kuijiefufafang and controlled by the medicine Changmei. We have observed the general condition and weight of rats and the pathological form of colonic mucosa. We also have studied the regulation to local inflammatory of colon and colonic smooth muscle movements and the differences of curative effect and different impact to the observed indicators between two drugs in different time and after treatment. We are looking for targets in the role of Chinese medicine, finding a theoretical basis of traditional Chinese medicine for anti-UC recurrence, establishing new ideas and methodsof Chinese medicine treatment for anti-UC relapse.MethodHealth-clean male SD rats were induced by TNBS/ethanol intermixture to UC models. They were divided into three groups randomly: model group, traditional Chinese medicine treatment group, and the control group of western medicine. In addition, we fed 30 normal rats for contrast. TCM treatment group were treated by the gavage with decoction of Kuijiefufafang, while the control group of western medicine were treated by gavage with the suspension of Olsalazine sodium (Changmei) as a positive control, the model group and the normal group were treated by gavage with distilled water as blank. We draw materials from the UC rats in 10 days after administration, 30 days after administration, and 10 days after drug withdrawal and observed following index: Rats general condition and weight; Changes in colonic mucosa in pathomorphology;changes of VIP in colonic mucosa; changes of SP in colonic mucosa; changes of NK-1R in colonic mucosa; changes of C-kit in colonic mucosa.Experiment Results:On the aspect of improving the living state, TCM treated group could has obvious effect since the acute period. And stopping pus and blood stool, improving independence activity, glossiness of coat emerged earlier than control group. Its effect could last to the chronic period and injury recovery period.As the observation of the local colon mucosa, both treated and control group had the effects of inhibiting the injury and and improving the plerosis of mucosa tissues. In acute period, effect of the former inferiored to the latter. But treated group’s effect of improving gt hyperblastosis and forming granuloma surpassed that of control group.Colon mucous VIP concentration in TCM treated group, control group and model group cut down at the time of treating 10 days and 30 days, but it in TCM treated group is significantly higher than that in control group at the same time. At the time of 10 days after drug withdrawal, colon raucous VIP concentration in TCM treated group is similar with normal group, and is higher than that in control group.Colon mucous SP concentration both in model group and control group are higher than normal, and it in TCM treated group is similar with normal group but significantly lower than control group at the time of giving medicine. There is no significant difference between two treated groups in Colon mucous SP concentration at the time of 10 days after drug withdrawal.Colon mucous NK-1R concentration in TCM treated group and control group are same with normal,but there is difference between them at time of 10 days after giving medicine. At the time of 30 days, Colon mucous NK-1R concentration in the two groups increased, but they cut down, and were similar with normal after drug withdrawal.Colon mucous C-kit concentration in normal group is not different with that in TCM treated group, but significant higher than that in control group at the time of 10 days after being treated. There is significantly difference between two treated groups in Colon mucous C-kit concentration at the time of 30 days and 10 days after drug withdrawal.Conclusion:The kuijiefufafang which based on treating with tonifying Qi and promoting blood flow and detoxifying Du has a goood effect on recovering normal condition , weight and colon musoca of UC rats. Among three periods, the effect of Kuijiefufafanf is better than Olsalazine sodium in stepping up VIP and cutting down SP of colon mucosa. In acute stage, Kuijiefufafang is better than Olsalazine sodium in cutting down NK-1R, but in chronic and recovery phase, they have the same effect. In acute stage, both treated groups are similar in Colon mucous C-kit concentration, and then Kuijiefufafang is more satisfact. Kuijiefufang based on treating with tonifying Qi, promoting blood flow and detoxifying Du ameliorated parasecretion of gastrointestinal peptide hormones, restrain localimmunity state, lessened mucous detriment of inflammation, adjusted smooth musle of colon. In conclusin, these contributions maybe one of reason about resisting relapse.