Dissertation
Dissertation > Environmental science, safety science > Processing and comprehensive utilization of waste > General issues > Wastewater treatment and utilization

Study of Treating Wastewater by Attapulgite Clay Immobilized Horseradish Peroxidase

Author GaoZuoJun
Tutor PengShuChuan
School Hefei University of Technology
Course Environmental Engineering
Keywords Horseradish peroxidase Attapulgite clay Immobilized Aniline Nitrophenol Enzyme kinetics
CLC X703
Type Master's thesis
Year 2010
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In this paper, attapulgite clay ( attapulgite ) as raw material , sodium silicate is kinked agent , starch as a pore-forming agent , study prepared for horseradish peroxidase immobilized composite attapulgite and impact the orthogonal experimental carrier dissipation rate of the various factors to determine the best carrier preparation conditions ; parallel to the single factor experiment to determine the best conditions of the immobilized HRP degradation of aniline and nitrophenol best conditions and immobilized HRP reuse performance; Lineweaver-Burk linear fitting relationship between the initial nitro phenol concentration and reaction speed , determine a the immobilized HRP degradation of nitrophenols the kinetic equation , the maximum reaction velocity Vmax and the Michaelis constant Km . Experimental results show that : attapulgite with the silicate and the starch mass ratio of 100:12:20 , granulated at 550 ° C muffle furnace in the calcination time is 2 hours , the fixing of HRP carrier with a high mechanical strength ; immobilized process conditions for the room temperature , fixed time 1.5 h , the amount of unit activity carrier 1.5 mg / U, pH = 5.0; immobilized enzyme degradation of aniline wastewater reaction , the reaction time of 1.5 hours , buffer system is not required to control the pH value of 100 mg per ml / L aniline wastewater needs 4.8U fixed enzyme , H2O2 aniline in a molar ratio of 1.2:1 ; HRP immobilized in this condition can be reused 4 times , and able to maintain more than 90% of the aniline removal rate . Immobilized HRP processing nitrophenol wastewater optimum conditions for the reaction time is 1.5 h per ml of 100 mg / L of nitrophenol wastewater takes 6.0 U to the enzyme activity ( calculated at a fixed rate of 95% , a fixed activity 5.7 U ), of H2O2 and nitro phenols preferred stoichiometric ratio of 1.2:1; immobilized HRP has better reusability , 7 times after repeated use , nitrophenols removal rate is still greater than 60% ; the immobilized HRP degradation of nitrophenols kinetic equation : 1 / v = 1.403 / [ S] - 0.081 , the maximum reaction speed Vmax = 12.35 mg / L · min, Michaelis constant Km = 17.32 mg / L .

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