Dissertation > Environmental science, safety science > Environmental pollution and its prevention > Soil Contamination and Control

Application of Endophytic Bacterium Isolated from Cd Hyperaccumulator Solanum Nigrum L for Phytoremediation Enhancement

Author WanYong
Tutor LuoShengLian;ZengGuangMing
School Hunan University
Course Environmental Engineering
Keywords Endophytes Hyperaccumulators Heavy metal Phytoremediation Detoxication
Type Master's thesis
Year 2009
Downloads 216
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Use of hyperaccumulating plants to extract heavy metals phytoremediation technology is one of the great potential of soil heavy metals pollution control technology . Phytoremediation main advantage is low cost , solar energy supply , in situ repair , retained less cultivated soil and secondary pollution . But at the same time to repair the effects of seasonal and long repair cycle and other issues , has become a \Therefore , it is necessary to carry out research to improve plant efficiency using microbial or chemical regulation . Plant endophyte and host plant synergy to improve the efficiency of phytoremediation is a promising program . Studies have shown that natural or engineered endophytes can enhance the repair capacity of the plant organic matter , but rarely role in heavy metal , the main reason is the lack of valuable strains , the main purpose of this paper is to self- hyperaccumulator plants screening of strains of plant growth promoting and heavy metal detoxification and investigated the effects of heavy metal phytoremediation . In this study, endophytes were isolated and purified strains LRE07 screened by the heavy metal cadmium (Cd) hyperaccumulator Solanum nigrum (Solanum nigrum L.) . 16s rDNA identified as Serratia . Investigated plant growth promoting relevant parameters found the strains can Nengchan indole acetic acid ACC deaminase and siderophore dissolved mineral phosphate capacity . These performance show that The endophytic bacteria LRE07 potential ability to promote plant growth amount . LRE07 on a variety of heavy metals have a high resistance , the minimum inhibitory concentration of Cd, Zn, Pb, Cu and Cr , respectively, 8mm , 5 mM , 4 mM , 10 mM and 12mM . Found in heavy metal ion detoxification experiments LRE07 selective heavy metal detoxification . The single ion conditions , it can bind more than 65 % of the Cd 2 < / sup> and 35% Zn 2 , while of Cu 2 , Cr < sup> 6 and of Pb 2 < / sup> did not remove the role of multi - ion system to get similar results . Implanted Solanum nigrum LRE07 back pot experiment results show that the biomass of Solanum nigrum have significantly increased , and Solanum nigrum tissues significantly increased Cd accumulation volume . This study demonstrated that endophytes and host plants plants collaborative relationship to improve the performance of phytoremediation is feasible .

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