cDNA Cloning, Tissue Expression and Bioinformatic Analysis of Fatty Acid Transport Protein 1 in Chicken
|School||Sichuan Agricultural University|
|Course||Animal Genetic Breeding and Reproduction|
|Keywords||chicken FATP1 expression IMF Bioinformatic|
In this study,total RNA was isolated from tissues of Tibetan and Sichuan Mountainous black-bone chickens(SMBC),Fatty Acid Transport Protein 1(FATP1) gene was proliferated used RT-PCR and the products sequences and amino structure were primary analyzed by bioinformatics.The developmental changes of FATP1 gene expression in SMBC usedβ-actin as internal standard control by the real-time quantitative RT-PCR method and the Correlation analysis between intramuscular fat(IMF), subcutaneous adipose thickness(SAT) and FATP1 mRNA expression.We compared the FATP1 gene expression difference in Tibetan,SMBC and Da Heng chicken(DH).The results are as follows:The coding sequence(CDS) of Tibetan and SMDC chicken FATP1 gene was 1941bp. By analysis of CDS:Between Tibetan and SMDC chicken,the homology rates of nucleotide sequences of coding region of FATP1 gene ware 100%respectively.Between products sequences and Gallus(XM423687),the homology rates ofnucleotide sequences of coding region of FATP1 gene were 99.9%respectively.There was a bases transition as G→A on site of 878 caused an amino transition as Arg→His on site of 293.There was a bases transition as A→G on site of 1614.Bioinformatic primary analysis suggested that chicken FATP1 is a plasma membrane protein which has one transmembrane helice and several hydrophobicity domains.The relative molecular weight of chicken FATP1 is 72.58 KDa,and isoelectric point is 9.08. The amino sequence may have signal peptide and phosphorylation sites.It has alpha helix, random helix and the similar Crystal structure of the Acyl-CoA synthetase.FATP1 mRNA tissues expression difference in SMBC suggested that FATP1 mRNA expression in muscle were significantly higher than that in brain,adipose tissues,liver and heart(P＜0.05) in embryonic phase.Then,the FATP1 mRNA expression in brain and adipose tissues grow fast.FATP1 mRNA expression in muscle and brain were significantly higher than that in adipose tissues,liver and heart(P＜0.05)in later growth phases.FATP1 mRNA expression in liver and heart were little low,and the expression in leg muscle was higher than that in pectoral muscle.FATP1 mRNA tissues expression developmental changes in SMBC suggested that FATP1 mRNA expression in different tissues has obviously development change in SMBC. FATP1 mRNA expression in brain and adipose tissues of 12 week were significantly higher than other weeks.FATP1 mRNA expression in liver and heart of 2 week and 12 week were significantly higher than other weeks.FATP1 mRNA expression in muscle of 2 week were significantly higher than other weeks.FATP1 mRNA expression in pectoral muscle and heart of SMBC were significantly higher than Tibetan at birth.FATP1 mRNA expression in pectoral muscle of DH were significantly higher than SMBC at birth.The correlation analysis showed that FATP1 mRNA expression in muscle and liver were found to be positively correlated with IMF(P＜0.05),but not correlated with SAT.We suggested that FATP1 mRNA expression could affect IMF significantly while the subcutaneous fat changes little.