Dissertation > Agricultural Sciences > Agricultural Engineering > Agricultural power,rural energy > Application of bio-energy ( biomass) > Biogas

Effect of Anaerobic Digestion on Indicative Bacteria in Cow Manure and Effect of Effective Microorganisms on Them during Anaerobic Digestion

Author SunCuiPing
Tutor ChaiTongJie
School Shandong Agricultural University
Course Preventive Veterinary Medicine
Keywords Cow Dung Anaerobic digestion Pathogenic microorganisms Beneficial microorganisms
CLC S216.4
Type Master's thesis
Year 2008
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China is the world largest producer of milk. With the continuous development of animal husbandry, Cow, feeding gradually dispersed to the specialized, intensive changes. This transformation of the mode of operation, has caused great difficulties to the health of dairy cattle manure treatment, causing serious environmental pollution. Manure contains a certain amount of pathogenic microorganisms, especially zoonotic disease pathogens. These pathogenic microorganisms caused the outbreak and spread of the disease through contaminated water, air and soil, and endanger the health of humans and animals. Livestock manure dirt harmless environmentally sound, resource handling and disposal requirements of these microorganisms effective disinfection treatment, in order to meet the environmental and ecological protection, the use of requirements, protect the environment and human health, and to promote the sustained and healthy development of animal husbandry itself. The anaerobic digestion of excreta disposal is an effective fecal pollution control and resource recycling technology, suitable for aquaculture in China lack of funds and shortage of resources of national conditions. Effective Microorganisms (Effective microorganisms) widely used in planting, breeding, and the field of environmental protection, has been systematically showing the good effect of promoting plant growth and improve livestock disease resistance, remove the fecal odor, improve the ecological environment and many other aspects and considerable application potential. In this study, household biogas digesters built by small farmers in Taian near sampling survey, a preliminary understanding of the anaerobic digestion of cow feces anaerobes, aerobes, fecal coliform and fecal streptococci content . The findings add beneficial microorganisms (Pseudomonas, Bacillus, lactic acid bacteria, yeasts and actinomycetes bacteria) on the the Cow Dung temperature (35 ℃) anaerobic digestion process of digestion system stability, under laboratory conditions, explore and indicator bacteria (fecal coliform bacteria, Clostridium perfringens, anaerobic and aerobic bacteria) to survive the impact of the situation. Small rural household biogas digesters processing of Cow Dung of its indicative microbial content of the survey results show that small digesters processing Cow Dung can significantly reduce cow feces aerobic, anaerobic bacteria, fecal coliform and fecal streptococci content, but has no effect on the content of the Clostridium perfringens. Although cows feces by the digesters processing major indicator bacteria levels significantly lower, the digested liquid out anaerobes, aerobes, still high levels of fecal coliform and fecal streptococci, reaching less than the national requirements livestock manure emissions standards. Therefore, there is a need to further improve the effects of the pathogenic microorganisms in the small digester processing cow feces. Mesophilic anaerobic digestion system in cow feces Add Pseudomonas, Bacillus, lactic acid bacteria, yeasts and actinomycetes bacteria can significantly promote the demise of fecal coliform bacteria in the digestive process, but in anaerobic and aerobic number did not change significantly in the whole digestive process. This may be due to the microorganism added late blooms increased due to the total number of bacteria in the digestive system. Clostridium perfringens not suited to the early stages of the digestive system environment, steady decline in the number of bacteria added, 10d, due to the formation of the adaptation to the environment, the number of basic no change. Beneficial microorganisms added the number of no effect. Add beneficial microorganisms can be improved acidogenic stage of acetate and butyrate production, suppressing the generation of propionic acid, favor the formation of a stable, good anaerobic digestion system. , NH4-N and pH in the anaerobic digestion process can inhibit the growth of pathogenic bacteria to some extent, but not the demise of pathogenic microorganisms.

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