Dissertation > Biological Sciences > Botany > Plant morphology

Ecological Anatomy and Comparative Embryology Studies on Swertia Bimaculata (Sieb.et Zucc.) Hook.f.et Thoms.ex C.B.Clarke and Tripterospermum Discoideum (Marq.) H.Smith (Gentianaceae)

Author LongHua
Tutor LiZuoMing
School Jishou University
Course Ecology
Keywords Swertia Hubei double butterfly Ecological Anatomy Embryology Tissue culture
CLC Q944
Type Master's thesis
Year 2008
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Swertia Hubei double butterfly are Gentianaceae plants in Western Hunan natural distribution area and habitats are very similar, but opposite evolutionary direction and use of resources way. For in-depth understanding of the system and the evolution of plant species formation, to adapt to the origin of species conservation biology has a certain theoretical and practical significance of these two plants, the ecological anatomy and comparative embryology research. Swertia Hubei double butterfly China's traditional Chinese medicine, medicinal value, but are suffering from severe stress, and so far from the point of view of the basic biology of the ecological anatomy, embryology research is still blank. Therefore, through this study, to further enrich perfect Swertia L. and double butterfly species, the basic theory of data services for the protection of traditional Chinese medicinal plants, development and sustainable use. . Ecological Anatomy of every part of the plant are coordinated together to accomplish a certain physiological functions. Studies have shown that different habitats Swertia, and Hubei dual butterfly stem, leaf anatomical structure to adapt to changes in the ecological environment. Among them, the most rapid change of the blade significantly strong light habitats, mainly as leaf thickness thickening to enhance the water retention capacity of the plant; gate sea than increased, in order to improve the photosynthetic performance and reflected glare irradiation; stomatal The density is increased, in order to increase the breathing area. Not only the anatomical structure of leaves will change, the anatomical structure of the stem will change accordingly, the stem cortical thickening to reduce water evaporation and improve the photosynthetic performance; developed pith favor the the plants water conservation and water storage capacity. In short, in the strong light habitats, plants on the one hand is to reduce water loss control transpiration, on the other hand to improve its photosynthetic performance. Embryology of Swertia Hubei double butterfly Embryology depth, comprehensive study. The main results are as follows: Swertia: anther room four anther wall development Basic; tapetum Shaped origin, belonging to glandular velvet carpet layer, the powder chamber has the degradation of tapetum Nuclear Early This layer cells have silk split convex medicine the center of the room and in situ degradation formed; intermediate cells layer; endothecium with epidermal persistent cell columnar elongation, fibrous thickening. Small meiosis type tetrad arrangement mainly tetrahedral shape, a small number of symmetrical shape and cross-shaped; mature pollen 2 - cell types. Ovary carpel room; Parietales tenuinucellate, a single bead, inverted curved ovule; megaspore mother cell meiosis form a linearly ordered four megaspore chalazal megaspore Featured embryo sac development Polygonum type. 3 persistent antipodal cells, each cell is multicore and abnormal enlargement of the antipodal cells of haustoria significantly, and to darker staining structure similar \Micropyle, fertilization, fertilization belong to the former type of mitosis. The endosperm development karyotype; the embryonic development eggplant type. Fruit ripening, seed development to the globular embryo stage. Antipodal cells persistent separatist in some Gentianaceae ephemeral plants in the important reproductive adaptation and evolutionary significance. The same time, based on available information, several plant Swertia comparative embryology research. Hubei Butterfly: anther four rooms; drug development dicotyledonous wall; the tapetum are profiled origin, cells with single-core, is a glandular tapetum, anther tapetum cells at the formation of placenta, and the rest parts of the the tapetal cells remains a layer of cells; anthers mature anther endothecium fibrous thickening and columnar elongation, epidermal cells reduced degradation, fibrous thickened obvious. Small meiosis type tetrad arrangement mainly tetrahedral shape, a small number of cross-shaped; mature pollen 2 - cell type. Ovary of 2 carpels; 1 room, Parietales, tenuinucellate single bead is anatropous ovule; ovule 4; the 4 megasporophyll meiosis to form large was linear arrangement, megaspore chalazal functional; embryo sac development Polygonum-type; the micropyle fertilization; The endosperm development karyotype; the embryo development eggplant type. Fruit ripening, seed development to the globular embryo stage. The same time, based on available information, several plant double butterfly genus comparative embryology research. 3 tissue culture research for Swertia Hubei double butterfly wildlife resources severely damaged, the paper systematically explored as a means for artificial propagation by tissue culture the tissue culture Swertia findings are as follows: the seed inoculation on induction medium, allowed to grow into seedlings whichever stems with buds, leaves and stems with buds as explants, add different hormone combinations on MS medium change training methods. In all of the experimental program, without the stems is the ideal explant material. Appropriate initial medium MS BA0.5mg / L sucrose, 3.0% proliferation medium was MS BA0.5mg / L IBA0.1mg / L sucrose, 3.0%, and root induction in 1/2MS NAA0.5mg / L 1.5% sucrose. The double butterfly tissue culture in Hubei results are as follows: to Hubei double butterfly stems with buds, stems with buds and leaves as explants, MS medium, by adding different hormone combinations, the establishment of Hubei double butterfly tissue culture micropropagation system. Stems with buds In all experimental program, callus highest rate, is ideal in vitro rapid propagation material. MS BA2.0mg / L sucrose, 3.0% more appropriate initial medium, proliferation medium was MS BA2.0mg / L NAA0.1mg / L sucrose, 3.0%, and root induction 1/2MS NAA0.5mg / L 1.5% of the sucrose medium was more appropriate.

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