Screening Molecular Targets of Phytophthora Sojae RXLR Effectors
|School||Nanjing Agricultural College|
|Keywords||Phytophthora sojae RXLR effectors hypersensitive response yeast two hybrid|
Phytophthora sojae (Kaufman & Gerdman) is an aggressive pathogen causing one of the most destructive diseases root rot on soybean,which is able to infect and kill soybean plants throughout much of the growing season worldwide. We bioinformatically identify almost 350 candidate RXLR effector genes in the genomes of Phytophthora sojae.These effectors can block plant disease resistance,improve the ability of pathogen infection and some effector proteins also has the eliciting function.Although their targets has not deeply researched.Current opinion on bacterial effectors’targets have been deeply researched,according to plant disease resistance signaling pathway is highly conserved,we cloned some researched bacterial effectors’targets,such as AtRin4, AtHSP90, AtSGT1b, AtRAR1, StR3a.By testing reporter genes in yeast two-hybrid system, we can find AtSGT1b and PsAvr1b-sp may interact with each other. Base on the above the findings, according to soybean’s genome sequence website and the NCBI website, we find 5 homology sequences with AtSG1b, GmSGT01 (Glyma01g43150.1)、GmSGT02 (Glyma16g29450.1)、GmSGT03 (Glyma09g23980.1)、GmSGT04 (Glymal0g37440.1)and NbSGT1.1.By using yeast two-hybrid technology again, GmSGT01, GmSGT03 may interact with PsAvr1b-sp.MAPK signaling pathway as a plant disease resistance signaling pathway has become an important part of various types of effectors’targets.We use potato virus vector PVX system, highly expressed a key kinase NbMKK1 (Nicotiana Benthamiana) of MAPK signaling pathway in tobacco cells can cause the HR response, but altogether expresses some effector molecules PsAvh331-sp, PsAvh94-sp and PsAvh328-sp may suppress the HR response which induces by it. By testing reporter gene and switch vector assay, we can find NbMKK1 and PsAvh94-sp may interact with each other. According to this research we conclude Phytophthora sojae effector Avh94 can block the signal pathway of plant immunity.