The Viral Etiology Study of Infantile Hepatitis Syndrome
|Keywords||Infant hepatitis syndrome Human cytomegalovirus EB virus Hepatitis B virus PCR-Southern blot|
Objective To investigate the human cytomegalovirus (Human cytomegalovirus, CMV), Epstein-Barr virus (Epstein-Barr virus, EBV), hepatitis viruses, including hepatitis A virus (Hepatitis A virus, HAV), hepatitis B virus (Hepatitis B virus, HBV) , hepatitis C virus (Hepatitis C virus, HCV), herpes simplex virus (Herpes simplex virus, HSV) infection in the pathogenesis of infantile hepatitis syndrome (infantilehepatitis syndrome) in children. By enzyme-linked immunosorbent assay (ELISA) method detected 47 cases of infant liver in children with hepatitis virus (anti-HAV, anti-HCV, hepatitis B 5) and HSV-IgM and HCMV-IgM and EBV VCA-IgM; specimens morning urine specimens for CMV genome quantitative detection; the PCR-Southern detect of peripheral blood of children and pharyngeal secretions EBVDNA. Results ① 47 patients HCMV DNA positive rate was 68.1% (32/47), HCMV-DNA was not detected in 40 normal children urine specimens, statistical analysis showed that: infant liver in children group HCMV-DNA positive rate higher than those in the control group, the difference was significant (x ~~ 2 = 43.08, P <0.01); 47 patients HCMV-IgM positive rate was 44.7% (21/47), and 40 normal control children HCMV-IgM test results was negative, statistical analysis showed that the infant liver in children group HCMV-IgM positive rate was significantly higher than that in the control group, the difference was significant (x ~~ 2 = 23.56, P <0.01); ② 47 cases of children with blood samples EBV DNA positive rate 19.1% (9/47), 40 cases of normal control children one cases of peripheral blood EBV DNA positive, the positive rate was 2.5%, the statistical analysis showed that: infant liver in children peripheral blood mononuclear cells EBV DNA positive rate was significantly higher than that control group, the difference was significant (adjusted x to 2 = 4.365, P <0.05); the pharyngeal secretion samples of 47 patients EBV DNA positive rate was 34.0% (16/47), 40 normal children one cases of pharyngeal secretions of EBV DNA-positive, the positive rate was 2.5% (1/40), children group pharyngeal secretions EBV DNA positive rate was significantly higher than that in the control group, differences have significant (x ~~ 2 = 13.67, P <0.01), 47 patients EBV VCA-IgM positive rate was 14.9% (7/47), 40 normal children EBV VCA-IgM test results were negative, statistical analysis showed that the infant liver in children group EBV VCA-IgM positive rate in the control group, the difference was statistically significant (x ~~ 2 = 4.62, P <0.05). (3), 5, 47 cases of children with hepatitis B detected only HBsAg positive cases of HBsAg and HBeAg positive, the infection rate was 4.3%. ④ 47 cases of infantile hepatitis syndrome in children with HSV-IgM, anti-HAV antibody and anti-HCV antibody tests were negative. (5) 47 cases of infant and liver in children 25 cases were cured, 15 cases improved, unchanged in 5 cases and 2 deaths. Conclusion HCMV infection is the main cause infant liver; EBV infection is the causative agent of some infantile hepatitis syndrome, Clinical younger children associated with liver dysfunction, the exclusion of intrauterine infection, especially cytomegalovirus, hepatitis B virus infection, and should take into account the possibility of EBV infection; infant liver HBV infection leads to decreased proportion of children.