Pharmacokinetics and Residues of Florfenicol in Yellow Catfish (Pelteobagrus Fulvidraco)
|School||Huazhong Agricultural University|
|Keywords||HPLC method Florfenicol Pharmacokinetics Remain Pelteobagrus|
Established high-performance liquid chromatography (HPLC) detection yellow catfish (Pelteobagrus fulvidraco) plasma and tissues florfenicol and florfenicol amine, and 25 ° C water temperature conditions single times mouth filling florfenicol in the health Pelteobagrus vivo pharmacokinetic characteristics and repeated oral administration residual eliminate the law. 1. Reversed-phase high performance liquid chromatography detection Pelteobagrus organization florfenicol and its major metabolite florfenicol amine content. Acetone extractant, n-hexane fat, acetonitrile - sodium dihydrogen phosphate buffer (30/70, V / V) as the mobile phase, detection wavelength of 225nm, column temperature 30 ° C, flow rate 1.0mL/min Agilent1200 high performance liquid chromatography detection. The results show that in the the 0.05-10.00μg/mL within the concentration range of the standard curve linear relationship. The average recovery of Florfenicol in various tissues florfenicol amine in the organizations of the average recovery of 81.4% to 89.0%, from 80.4% to 85.7%. Florfenicol and Florfenicol amine detection limit of the organizations were: plasma 0.025μg/mL, 0.015μg/mL; muscle 0.015 μg / g, 0.01Oμg / g; skin 0.030μg / g 0.020μg / g; liver 0.025μg / g, and 0.025μg / g; kidney 0.050μg / g, and 0.025μg / g. Established detection method can detect Florfenicol and Florfenicol amine content in fish tissue. 2 25 ° C water temperature conditions, after a single oral administration of the dose to 10mg / (kg.bw), florfenicol and florfenicol amine in yellow catfish in vivo pharmacokinetics and tissue distribution characteristics. The results show that in the plasma, muscle, skin, florfenicol and its metabolite florfenicol amine drugs - when curves are in line with the one-compartment model; liver and kidney, florfenicol and its metabolites fluorophenyl florfenicol amine drugs - curve in line with a two-compartment model, blood in the apparent volume of distribution (Vd / F) for 2.52L/kg drugs in the body of yellow catfish are widely distributed. The peak time in the organizations of the liver (1.82h) lt; kidney (2.26h) lt; skin (6.15h) lt; muscle (6.32h), show that the rapid accumulation of drugs in the liver and kidney. The organizations florfenicol amine Cmax compared with florfenicol low, indicating that the and Florfenicol in yellow catfish in vivo elimination of unchanged drug metabolism. In this experiment, florfenicol maximum plasma concentration in vivo Pelteobagrus 2.35μg/mL, with most aquatic pathogens minimum inhibitory concentration (MIC) ratio greater than 4, indicating that florfenicol plasma concentration in vivo Pelteobagrus inhibitory effect can be achieved. 3 to 10mg / (kg.bw) · d dose continuous oral administration 3d, florfenicol and its major metabolite florfenicol amine residues in skin muscle law. The majority of countries in the international for florfenicol drug residues provisions: the Florfenicol its major metabolite florfenicol amine sum 1mg/kg. The experimental results show that, 1d after administration in the edible tissue of catfish and the average total concentration of both 3.39μg / g, higher than 1μg / g after administration 3d experiment measured individuals fluorine benzene florfenicol and florfenicol amine total concentration of 0.5μg / g, lower than the maximum residue limits of 1μg / g, the total concentration of all individuals of florfenicol and florfenicol amine 5d 0.062μg / g lower than the maximum residue limits of Japan 0.1 μg / g, 7d both undetectable. Therefore, the recommended dose of 10mg / (kg.bw) · d in 25 ° C water temperature conditions, multiple oral administration, withdrawal period (WDT) not less than 3d. To achieve Japan under the maximum residue limits of 0.1 μg / g, the withdrawal period (WDT) not less than 5d.