Studies on Population Structure of Rhytismataceae from Wanfoshan National Forest Park and Its Sequence Analysis of Two Related Lophodermium Species
|School||Anhui Agricultural University|
|Keywords||Stains discomycetes Division Classification Morphology and Anatomy ITS sequences Kinship|
In this paper, a more advanced fungi histological classification technology and multi-shape analysis methods, from the morphological and anatomical ontogeny, ecological and geographical distribution of traits training, on the the Wanfo National Forest Park stains discomycetes Division members more systematic classification. Speckle shell two similar species of the genus the the (pine needles Speckle shell Lophodermium pinastri and Qiao speckle shell L. pini-excelsae) 13 strains of rDNA-ITS sequences were amplified by PCR with determination, with GenBank, already sequence homology analysis; phylogenetic tree was constructed by MEGA neighbor-joining method (neighbour-joining method, NJ). Some morphological characteristics and host species differences in the the the class fungi species on the level of classification of the importance to shed further light on the genetic diversity and relationship of the two kinds of intraspecific and interspecific. -Darker \area stains sclerotiorum Division fungi classified research. Total identify the part of the toothed genus Coccomyces, speckle shell case Lophodermium, the Hypoderma Hypoderma, the the small shoes spore disk genus Soleella, Trier disk genus Terriera 5 genera and 11 species, including a new record is terriera new species T.huangshanensis new record species T.minus; and nine domestic known species additional features to perfect its morphological structure, ontogeny, WORM their hosts The new record new distribution and geographic. Morphological and anatomical characteristics are provided to various descriptions and illustrations, host and habitat records, and discussed its of similar taxa, parasitic, and pathogenic. Compiled binary Key to Species. Former lip Trier plate the genus Terriera with the speckle the shell case Lophodermium the difference is that the missing child seat covering a strength carbonation, no obvious structural extension material, more with a pale beneath a thin layer of thin silk tissue covering layer the adjacent base layer with a nearly colorless or pale taupe moment cellular tissue, or angle Cellular organization, paraphyses enlargement, often at the top of branches, sometimes intertwined or bond and the formation of the capsule layer is. With Hypoderma Hypoderma difference is that the latter lip usually well developed, rare swelling of the top of the side of the wire, ascospores generally oval-shaped, cylindrical to clavate. Born the the on the hair sticks grid drug Eurya deciduous Huangshan Trier sclerotiorum T. huangshanensis ZZYang, YR Lin CL Hou its longitudinal seam on each side of a strong carbonation no obvious structural extension thereof; cover layer and the base layer adjacent at the horn tissue underdeveloped; lip missing and other characteristics included Terriera case. The members of this genus, T. huangshanensis very special because it's the same period in mature asci mature hymenium often break through the cover layer, and even scale up to and even beyond the top of the cover layer, so as a new species. The results show: morphology and anatomy, L. pinastri addition to the child seat base layer, lined and pycnidium of opening characteristics, the remaining important characteristic of L. pini-excelsae has a lot of similarities. Of L. pinastri the ITS sequences the GC average content (51.5%) than L. pini-excelsae average GC content (50.1%). As can be seen from the phylogenetic tree, L. pinastri and L. pini-excelsae 13 strains constitute two separate evolutionary branches I and II, the branch I by two small branch of the A, B, branch A by parasitic Scots pine and fellow European native trees on the European black pine 7 L. pinastri strain. L. pinastri parasitic Akamatsu and one thousand Akamatsu on branch B, strains priority together. Evolutionary branch II by the Japanese five needle pine and Armand the two L. pini-excelsae strains were isolated from Japanese five needle pine and Armand series single vascular bundle Songya five-needle pine group of plants, and evolutionary branch I the host genus of P. tabulaeformis group plants for double vascular bundles Songya. It can be seen, the host of genetic diversity within species also have a more important impact origin intraspecific genetic differences no significant correlation. ITS sequence analysis as to distinguish between two similar species is an effective means.