Dissertation > Agricultural Sciences > Forestry > Forest Protection > Forest Pest and Disease Control > All kinds of tree pests and diseases and their prevention

Physiological and Biochemical Response to Poplar Canker Toxin in Callus Tissue of Poplar

Author WangHaiYan
Tutor ZhangRuLi
School Gansu Agricultural University
Course Forest Management
Keywords Poplar Callus Physiological and biochemical Ulcer disease Toxin
CLC S763.7
Type Master's thesis
Year 2010
Downloads 51
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{Of Botryosphaeria dothidea in (Moug. Ex Fr.) CES poplar canker de Not, Dothiorella gregaria, Sacc} is a devastating disease of poplar and difficult to control. In this study, tissue culture techniques poplar callus induction, and thus in four generations toxin, were measured and analyzed for each generation survival somaclonal: determination of peroxidase (POD) activity, superoxide dismutase (SOD) activity of phenylalanine ammonia-lyase (PAL) activity, polyphenol oxidase (PPO) activity, chitinase (Chitinase) activity of these physiological and biochemical indexes. To reveal the most important factor in Poplar anti-ulcer disease, the results show that: (1) Populus callus treated with toxins, in each generation processing, enzyme activity has been some changes, the trends are basically first increased After the fall: callus PAL (PAL) activity increased rapidly at first, after becoming more and more gentle decline with the progression of the disease, and PAL activity (9.8) than before (2.3) increased 5 times; POD activity in the third generation reaches the maximum ratio without toxin treated callus POD activity was increased 2-fold; SOD activity of the process of induction of maximum value of the third generation (1964.914) than the control (1203.693) an increase of 1.6 times; the callus induced PPO activity in the first generation to the fourth generation is always higher than that of the control, and the maximum peak of activity in the third generation after infection; of the Chitinase activity with the extension of time in the sampling also obvious change, and the second generation of the callus enzyme activity reached a peak, the activity rate of change of 135%, and 62% in the fourth generation activity changes remained. As can be seen from these results, Populus alba callus after toxin treatment, increased the activity of various enzymes, and enzyme activity with the treatment time, the overall higher than the callus without toxin treatment (control) enzyme activity. From this we can see, callus after four generations of processing, the survival of somatic cells has gained a certain amount of resistance against the infringement of toxins and laid the foundation for the cultivation of anti-the poplar canker new varieties. (2) the detection of several protective enzymes, despite all the activity in the induction treatment change, but the induction treatment within four generations, protective enzymes are a lot of changes. SOD, PPO and POD reached the maximum activity in the induction of the third generation of the processing; measured five kinds of protective enzymes have shown a similar law is first increased and then decreased; while some protective enzymes are not synchronized, which may be related to The respective effects of differences in plants. (3) from the measured physiological and biochemical indexes, callus after pathogen infection, pathogens activated poplar callus a variety of defense responses in vivo, including the rapid increase in PAL, PPO, POD activity, SOD and Chitinase a large number of synthesis, increased the strength of the cell wall, so that the poplar callus induced resistance to canker local resistance signal in the induction treatment, the resulting system of ulcer disease induced resistance.

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