Research on the Effects of Different LED Lights on Physiological and Biochemical Characteristics of in Vitro Grape Plantlets
|School||Gansu Agricultural University|
|Course||Crop eco- physiological|
|Keywords||LED Light quality Photoperiod Grape plantlets in vitro Growth dynamics Leaf pigment Stoma Enzyme activity Photosynthetic rate|
In plant tissue culture, photosynthetic photon flux density (PPFD: Photosynthetic Photon Flux Density), photoperiod and spectral distribution built on plant photosynthesis and morphology play an important role. Plant tissue culture mainly rely on the electric light, traditional electric light on the the biotic energy efficiency, heat, light, electricity accounts for about 65% of the entire cost of electricity, is one of the plant tissue culture in higher non-labor costs. Therefore, plant tissue culture, a new type of LED light source, the regulation of light quality and PPFD not only be able to regulate tissue culture plant growth and development and built form, and can significantly reduce energy consumption and reduce costs. While addressing the the impure light quality problem, increase the reliability of test results. This study the the grape rootstocks Beida (Beta) and variable leaf grapes (Vitis piasezkii Maxim) plantlets were investigated in LED white, red, yellow, blue, green, and blue and red six light quality, 24 h · d-1 for 16 h · d-1 for 12 h d-1, 8 h · d-1 four photoperiod plantlets growth and development of dynamic ground site (plant height, leaf area, biomass, section length, stem diameter) and underground parts (root, root number, root length, root diameter) proliferation and pigment content, porosity, activity effect. Tissue culture in order to get the best LED light quality, photoperiod. Achieved major findings: (1) LED red light is conducive to grape plantlets above and below ground parts of the longitudinal growth; LED Blu-ray suppression grape plantlets in vitro above and below ground parts of the longitudinal growth, promote lateral growth; LED blue red grapes plantlets photosynthetic rate than ordinary fluorescent. Red, blue and blue red significant role in promoting the increase of biomass under 16 h · d-1: 24 h · d-1, Blu-ray and blue red light crude internodes. (2) LED yellow, green, light cycle of 16 h d-1, 12 h · d-1 light is conducive to an increase in plant height, blue and blue red light is conducive to the test tube at 16 h · d-1 under increase in seedling leaf area, green and yellow in the next 24 h · d-1 leaf area are small. Section length green and yellow longer under blue shortest; 8 h · d-1, green and yellow lower internodes finest; plantlets in 16 h · d-1, yellow under thick finest, and photosynthetic rate in the LED blue red LED yellow and green negative. (3) are demonstrated in the type of LED light quality and light cycle, the greater the degree of stomatal frequency, the the stomatal length × width smaller the different genotypes of the desired light quality, photoperiod are not identical, variable frequency grape leaf stomata degree in Blu-ray, 16 h · d-1 under high Beida plantlets stomatal frequency in red, blue, and 24 h · d-1 under the maximum. Red, blue and blue red conducive to the synthesis of chlorophyll, 8 h · d-1 compared with the other three photoperiod chlorophyll synthesis capacity is poor, and the light quality and photoperiod effect due to genotype differences in SOD activity in the blue the next highest, the lowest under red light. Beida plantlets 8 h · d-1 under the maximum 24 h · d-1 under the minimum, variable leaf grape plantlets in vitro in 16 h · d-1 under the maximum 12 h · d-1 the lowest. Plantlet PPO, POD and CAT activity strength are: 24h.d-1 GT; 16 HD-1 GT; 12 HD-1 GT; 8 hd-1.