In Vitro Rapid Propagation and Its Cell Histological Observations of Siraifia Grosvenorii (Swingle) C.Jeffery
|School||Fujian Agriculture and Forestry University|
|Keywords||Mangosteen In vitro culture Anatomy Adventitious buds Adventitious roots|
Of the test material Mangosteen rapid propagation in vitro culture of shoot tips, stem, leaf and hypocotyl substandard implant the start growth, proliferation of plantlets and shoots rooting stage of organogenesis cell histology observed . The main results are as follows: 1. Mangosteen shoot tip culture. Peel fruit, melon Han, Jane fruit One variety of shoot tip explants, to explore the Mangosteen different varieties in vitro culture seedling method. The results show that: the the meristem culture medium to MS 0.5mg · L-1 6-BA 0.5 mg · L-1IAA the solid medium is preferred; peel fruit, melon varieties of Han the induction ratio Jane fruit One is better; strains. Department of the establishment with the postponement of the vaccination period, the survival rate was linearly decreasing change, the sooner the higher the survival rate of vaccination. 2 the Mangosteen shoot tip virus-free training and its histological observation. Mangosteen shoot tips of the Ad Mangosteen bud induction, BA shoot tip culture Shoots, different sugar concentrations Shoots and detoxification treatment using shoot tip culture combined with heat treatment; Mangosteen shoot tips in vitro culture process histological observation. The results show that The Mangosteen shoot tip virus-free training to MS 1 mg · L-1 6-BA 1 mg · L-1IAA add 3% sucrose solid medium is preferred; the shoot tip virus-free culture terminal bud growth, axillary buds and adventitious buds of three ways. The terminal bud growth and plant regeneration. 3 Mangosteen stem culture. Section to the Mangosteen Qingpiguo young shoots as explants, the degree of development of the comparison section bud induction. The results show that, within Section 3-14 of the young shoots, the higher the degree of differentiation, the higher the budding rate, the higher the rate of pollution; 7-10 callus highest degree. 4 hypocotyls culture and histological observation. Hypocotyls explants, explore a different medium Anatomical observation of the impact of of hypocotyl adventitious buds regeneration, regeneration of adventitious buds and somatic embryogenesis. The results showed that hypocotyl modified MS medium supplemented with 4 mg · L-1 6-BA 1 mg · L-1IAA solid medium, an average of up to 7.6 adventitious buds / explant. The anatomical structure of adventitious buds and somatic embryos originated in hypocotyl callus surface. Mangosteen blade culture and histological observation. Mangosteen leaves as explants to explore different basic medium, different inoculation methods, the effects of different plant growth regulators on adventitious buds, and adventitious buds formed cell histology observed. The results show that the basic medium to improve MS is more appropriate, additional 1 mg · L-1 6-BA 0.5 mg · L-1NAA 0.5 mg · L-12 ,4-D of the solid medium, averaging more blocks of renewable shoots up to 5.6. Front of the blade contact medium the way bud differentiation rate of 90.3%. Silver nitrate on adventitious buds with obvious effect, the differentiation rate of 86.7%. Adventitious buds originated in the the leaves close callus surface. 6 plantlets proliferation and cell histological observation. Mangosteen plantlets, compared placed Shoots 6-BA on the proliferation of cultured cell histology observed the same time proliferation of Luohanguo internodes culture. The results showed that the festival culture bud differentiation was higher than that in the section between the training methods; stem segments of medium to MS 0.6 mg · L-1 6-BA 0.2 mg · L-1IBA 0.2 mg · L-1IAA, additional 3% sucrose solid culture base is better the axillary buds plantlets proliferation. The callus not normally produce adventitious buds. 7 rooting and its histological observation. Mangosteen plantlets as test materials, a different way of rooting comparison test, and the rooting process Morphological Anatomy. The results showed that the: Mangosteen plantlets stem segment base adventitious roots originated in the phloem and beam forming layer cross-site. Section of the Ministry of adventitious roots originated in the medullary rays and beam forming layer cross the site, belong to the origin. Section of the Ministry of adventitious roots close to the leaf gap, but also with the leaf trace root primordia early. Section of the Ministry of section rooting early root good root distribution structure.