Superparamagnetic contrast agents ferrite nanoparticles research
|School||Shenyang Pharmaceutical University|
|Keywords||Ferrite colloidal superparamagnetic Injection of superparamagnetic ferrite nanoparticles Physicochemical properties Preliminary stability Security Pharmacokinetics Pharmacodynamic|
Superparamagnetic ferrite nanoparticles (SPIO, Superparamagneitc Iron Oxide) is a new magnetic resonance imaging contrast agents (Magneitc Resonance Imaging Contrast Agents) magnetic active substances can be used to develop the reticuloendothelial system targeted MRI contrast agent. Has an imaging effect, high security features. To further expand the MRI contrast agents in the domestic clinical applications, this paper was prepared for injection ferrite superparamagnetic nanoparticles. First, FeCl 2 and FeCl 3 as raw materials, chemical coprecipitation of SPIO colloid established phenanthroline colorimetric determination of total iron colloids content. Colloidal appearance, reaction yield and g magnetization of evaluation indicators, on the basis of single factor tests conducted L 9 (3 4 sup>) orthogonal test the preparation process and prescription, the optimal preparation obtained colloid yield of 49.3% total iron, g magnetization 0.64emu / g (n = 3). SPIO colloid in the preparation, based on the made into lyophilized. Compare the flow steam sterilization method, microporous membrane sterilization method, both of which had no effect on the quality of colloidal choose simple sterilization method microporous membrane as a colloidal method of sterilization. Conductivity Method using freeze-dried proppant after adding different colloidal eutectic point. According to the appearance and lyophilized preparation reconstituted situation, choose 2% mannitol as lyophilized proppant lyophilized best results. Injection of superparamagnetic ferrite nanoparticles preliminary stability test shows: preparations at high temperatures, bright light are stable, no significant change. And RH75% at 40 ℃ under the conditions of accelerated stability test preparation is expected to be valid up to two years. For injection of superparamagnetic ferrite nanoparticles physicochemical properties were studied: lyophilized powder reconstituted TEM images proved chemical coprecipitation superparamagnetic nanoparticles ferrite particles of iron oxide body nuclei is about 3Onm. Laser scattering method formulations average particle size of 98.7nm. X-ray powder diffraction and electron diffraction analysis further confirmed the prepared colloid containing Fe 3 O 4 , Fe 3 O 4 crystal particle size is small, so that X-ray diffraction peak broadening. Using a vibrating sample magnetometer measuring magnetic properties of the preparation, to demonstrate that their superparamagnetic. For injection of superparamagnetic ferrite nanoparticles were investigated safety, rabbit ear vein irritation test, eye drops irritation and hemolysis experimental results show that: the preparation irritation. In Shenyang Pharmaceutical University Thesis Chinese Abstract mice LD5ofl77ITlg / k. Conducted a preliminary in vivo rat pharmacokinetic experiments using five seven rPADAP02 +5- Bin 2 knives steep azo BU 5 - delete diethylaminobenzaldehyde pseudo chromogenic agent, the use of non-ionic surfactant OP in micellar solubilization spectrophotometry Determination of trace iron in plasma, pharmacokinetics features consistent with double compartment model. Tumor-bearing mice using h-weighted magnetic resonance imaging in vivo to study the injection of superparamagnetic ferrite nanoparticles as contrast agents for imaging Mill enhancement effect, liver and liver tumor tissue measurements showed that: before and after administration, liver Ministry normal tissue signal intensity decreased o <0 Knife 5 BU and tumor tissue signal intensity change, the contrast agent can enhance the imaging results. 30min after the administration of optimal MRI imaging results.