Dissertation
Dissertation > Medicine, health > Surgery > Traumatology > Burns and scalds ( burns )

The cytokines, rare earth, Tetrandrine the experimental study of epidermal cell growth and apoptosis

Author WangXiZuo
Tutor ChenGuoAn;LiGuoHui
School Jiangxi Medical College
Course Burn Surgery
Keywords TNF - alpha NGF IGF Rare Earths yttrium Tetrandrine Epidermal cell growth and apoptosis Affect
CLC R644
Type Master's thesis
Year 2001
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Objective: To explore the cytokines TNF-alpha, NGF, IGF-1 and rare earth (yttrium chloride), Tetrandrine epidermal growth and apoptosis of the purpose of wound healing, dermatology new drug of choice basis. : 1, using trypsin digestion were isolated rat neonatal rat epidermal cells. 2, the establishment of UV-induced epidermal cell apoptosis model, using electron microscopy, flow cytometry and TUNEL assay cell apoptosis, to determine ultraviolet radiation and apoptosis relationship. 3, the number of cells 1 × 10 5 / hole inoculated with a 96-well culture plate, divided into six groups, each group were added to two different concentrations of TNF-alpha, NGF, IGF and rare earth (yttrium chloride), Tetrandrine the control group, normal saline, 72 hours, epidermal cell viability was measured by MTT assay and TUNEL assay epidermal cell apoptosis rate. 4, the cell number of 1 × 10 ~ 5 / hole inoculated to a 96-well culture plate, and packet Ibid. Cells were cultured for 72 hours after the UV irradiation of epidermal cells, and then each group were added to two different concentrations of TNF-alpha, NGF, IGF and rare earth (yttrium chloride), Tetrandrine the control group, normal saline, cultured for 48 hours The cells were collected, epidermal cell viability and TUNEL assay epidermal cell apoptosis rate was detected by MTT. Results: 1 UV irradiation can induce epidermal cell apoptosis, TUNEL method and flow cytometry results are generally the same. 2, NGF, IGF promote epidermal cell growth and survival, the role of IGF greater than NGF, and more significant role of epidermal cells after UV irradiation. \cell growth impact of seven independent of the concentration. 3 * N Bu, rare earth (chlorinated button * powder anti-alkali to promote epidermal cells withered 0 ': more significant role in the normal epidermal cells. 1 * F * GF inhibition of epidermal cells apoptosis, more significant role of epidermal cells after irradiation of various factors on epidermal cell apoptosis and concentration independent. Cheng (Conclusion: 1 gas ultraviolet radiation can induce apoptosis of epidermal cells; 2,1 * F ** F promote epidermal cell growth, inhibition of epidermal cells withered L: role of mr * NGF has a significant protective effects on epidermal cells after UV irradiation: TNF Q (L Chloride Fei powder anti-c alkali dilute inhibition of epidermal Programme extracellular Niu K, to promote epidermal Shenzhen apoptosis, the role of the order of * N Bu u, Tetrandrine, rare earth (chlorinated drilling), K on the small normal epidermal cells is more pronounced.

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